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. 1987 May;6(5):1301–1307. doi: 10.1002/j.1460-2075.1987.tb02368.x

Localization of the mcf.2 transforming sequence to the X chromosome.

T Noguchi, M G Mattei, I Oberlè, J Planche, J Imbert, C Pelassy, F Birg, D Birnbaum
PMCID: PMC553933  PMID: 3038515

Abstract

A transforming sequence was identified using co-transfection of DNA from the human mammary carcinoma cell line MCF-7 and of a G418 resistance gene into NIH 3T3 cells, followed by tumor formation in athymic mice. This sequence, named mcf.2, was molecularly cloned. A transforming activity resides in a cosmid clone of 42 kb. mcf.2 did not cross-hybridize with the known oncogenes tested. In situ hybridization localized it on the X chromosome, probably at q27. This localization was confirmed by hybridization to a panel of human--rodent cell line DNAs.

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