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GAPDH gene knockdown efficiency was validated in lung adenocarcinoma cells (A549) after 24 h treatment with micelleplexes assembled via batch reactor and microfluidic mixing at an N/P ratio of 6 with 50 pmol hGAPDH siRNA. GAPDH expression was normalized with β-actin expression and quantified by real time PCR. Data points indicate mean ± SD (n = 3). One-way ANOVA, *P < 0.01.
