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. 1985 Jan;4(1):17–23. doi: 10.1002/j.1460-2075.1985.tb02311.x

Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays

Andy Pereira 1, Zsuzsanna Schwarz-Sommer 1, Alfons Gierl 1, Isolde Bertram 1, Peter A Peterson 1, Heinz Saedler 1
PMCID: PMC554145  PMID: 15915572

Abstract

A newly isolated, unstable mutation wx-844::En-1 of Zea mays was proven to be caused by the insertion of the autonomous transposable element En into the Waxy (Wx) gene. Molecular analysis revealed that En-1 is 8.4 kb long, has a 13-bp long perfect inverted repeat at its termini and generates a 3-bp target site duplication. En-1 is integrated into an intron located approximately in the middle of the transcribed region of the Wx gene. Structural evidence is presented indicating that a receptor component (Inhibitor) can arise by internal deletion of an autonomous En element.

Keywords: autonomous element, receptor element, waxy locus, genetic analysis, sequence analysis

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Selected References

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