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. 1985 Feb;4(2):351–359. doi: 10.1002/j.1460-2075.1985.tb03636.x

Consequences of frameshift mutations at the immunoglobulin heavy chain locus of the mouse.

B Baumann, M J Potash, G Köhler
PMCID: PMC554193  PMID: 3926482

Abstract

From an IgM secreting hybridoma line we have isolated 16 spontaneous mutants that produce truncated IgM polypeptides. The size of the mu-mRNAs produced by these mutants is normal, but they express 3- to 100-fold less mu-mRNA and mutant mu-protein than the parental cell line. Nucleotide sequence analysis of cloned mu-genes and/or their mRNAs show frameshift mutations that generate in-phase chain termination codons. The extent of the reduction in mu-mRNA levels depends on the position of the nonsense codon within the gene.

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