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. 1985 May;4(5):1273–1277. doi: 10.1002/j.1460-2075.1985.tb03772.x

Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense.

T Baltz, D Baltz, C Giroud, J Crockett
PMCID: PMC554336  PMID: 4006919

Abstract

A semi-defined medium for the cultivation of bloodstream forms of the African trypanosome brucei subgroup was developed. Out of 14 different strains tested, 10 could be cultured including Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense. The presence of a reducing agent (2-mercaptoethanol or thioglycerol) was found to be essential for growth. The standard medium consisted of Hepes buffered minimum essential medium with Earle's salts supplemented with 0.2 mM 2-mercaptoethanol, 2 mM pyruvate and 10% inactivated serum either from rabbit (T. brucei, T. equiperdum, T. evansi and T. rhodesiense) or human (T. gambiense). Although a general medium could be defined for the long-term maintenance of trypanosome cultures, the initiation to culture nevertheless required particular conditions for the different strains. The cultured trypanosomes had all the characteristics of the in vivo bloodstream forms including: morphology, infectivity, antigenic variation and glucose metabolism.

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Selected References

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  1. Afchain D., Le Ray D., Van Meirvenne N., Fruit J., Capron A. Analyse immunoélectrophorétique comparative des formes de culture et des formes sanguicoles de Trypanosoma (Trypanozoon) brucei gambiense. Caractérisation d'antigènes spécifiques de stade et de type antigénique. Ann Immunol (Paris) 1975 Jan;126(1):45–50. [PubMed] [Google Scholar]
  2. Arrick B. A., Griffith O. W., Cerami A. Inhibition of glutathione synthesis as a chemotherapeutic strategy for trypanosomiasis. J Exp Med. 1981 Mar 1;153(3):720–725. doi: 10.1084/jem.153.3.720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Balber A. E. Primary murine bone marrow cultures support continuous growth of infectious human trypanosomes. Science. 1983 Apr 22;220(4595):421–423. doi: 10.1126/science.6836284. [DOI] [PubMed] [Google Scholar]
  4. Brun R., Jenni L., Schönenberger M., Schell K. F. In vitro cultivation of bloodstream forms of Trypanosoma brucei, T. rhodesiense, and T. gambiense. J Protozool. 1981 Nov;28(4):470–479. doi: 10.1111/j.1550-7408.1981.tb05322.x. [DOI] [PubMed] [Google Scholar]
  5. Capbern A., Giroud C., Baltz T., Mattern P. Trypanosoma equiperdum: etude des variations antigéniques au cours de la trypanosomose experimentale du lapin. Exp Parasitol. 1977 Jun;42(1):6–13. doi: 10.1016/0014-4894(77)90055-8. [DOI] [PubMed] [Google Scholar]
  6. Fish W. R., Looker D. L., Marr J. J., Berens R. L. Purine metabolism in the bloodstream forms of Trypanosoma gambiense and Trypanosoma rhodesiense. Biochim Biophys Acta. 1982 Nov 24;719(2):223–231. doi: 10.1016/0304-4165(82)90092-7. [DOI] [PubMed] [Google Scholar]
  7. Hill G. C., Shimer S., Caughey B., Sauer S. Growth of infective forms of Trypanosoma (T.) brucei on buffalo lung and Chinese hamster lung tissue culture cells. Acta Trop. 1978 Sep;35(3):201–207. [PubMed] [Google Scholar]
  8. Hirumi H., Doyle J. J., Hirumi K. African trypanosomes: cultivation of animal-infective Trypanosoma brucei in vitro. Science. 1977 May 27;196(4293):992–994. doi: 10.1126/science.558652. [DOI] [PubMed] [Google Scholar]
  9. Lumsden W. H., Herbert W. J. Pedigrees of the Edinburgh Trypanosoma (Trypanozoon) antigenic types (ETat). Trans R Soc Trop Med Hyg. 1975;69(2):205–208. doi: 10.1016/0035-9203(75)90156-x. [DOI] [PubMed] [Google Scholar]
  10. Magnus E., Vervoort T., Van Meirvenne N. Serological cross-reactions among trypanosome variable antigen isotypes of the subgenus Trypanozoon. Ann Soc Belg Med Trop. 1982 Mar;62(1):25–39. [PubMed] [Google Scholar]
  11. Meshnick S. R., Blobstein S. H., Grady R. W., Cerami A. An approach to the development of new drugs for African trypanosomiasis. J Exp Med. 1978 Aug 1;148(2):569–579. doi: 10.1084/jem.148.2.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nantulya V. M., Doyle J. J. Stabilization and preservation of the antigenic specificity of Trypanosoma (Trypanozoon) brucei variant specific surface antigens by mild fixation techniques. Acta Trop. 1977 Dec;34(4):313–320. [PubMed] [Google Scholar]
  13. Rifkin M. R. Identification of the trypanocidal factor in normal human serum: high density lipoprotein. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3450–3454. doi: 10.1073/pnas.75.7.3450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Riou G., Baltz T., Gabillot M., Pautrizel R. Absence of kinetoplast DNA in a late antigenic variant of Trypanosoma equiperdum. Mol Biochem Parasitol. 1980 Apr;1(2):97–105. doi: 10.1016/0166-6851(80)90004-3. [DOI] [PubMed] [Google Scholar]
  15. Vickerman K. Antigenic variation in trypanosomes. Nature. 1978 Jun 22;273(5664):613–617. doi: 10.1038/273613a0. [DOI] [PubMed] [Google Scholar]

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