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. 1985 May;4(5):1345–1349. doi: 10.1002/j.1460-2075.1985.tb03783.x

Site-specific DNA binding by the bacteriophage SP01-encoded type II DNA-binding protein.

J R Greene, E P Geiduschek
PMCID: PMC554347  PMID: 4040015

Abstract

The bacteriophage SP01 genome encodes a virus-specific type II DNA-binding protein, TF1. The bacterial proteins of this ubiquitous and evolutionarily conserved class are thought to bind non-specifically to DNA. In contrast, the experiments described here demonstrate that TF1 binds to specific sites in SP01 DNA. Several of these sites have been characterized by DNase I 'footprinting' and four of them have been shown to overlap strong phage promoters for Bacillus subtilis RNA polymerase holoenzyme. We speculate on the possible structural basis of site-selective DNA binding by a protein of this class.

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