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. 1985 Jul;4(7):1813–1818. doi: 10.1002/j.1460-2075.1985.tb03855.x

Antibodies against synthetic peptides react with the second Epstein-Barr virus-associated nuclear antigen.

J Dillner, B Kallin, G Klein, H Jörnvall, H Alexander, R Lerner
PMCID: PMC554422  PMID: 2992945

Abstract

Five peptides were synthesized on the basis of amino acid sequences predicted from the transformation-associated BamHI WYH region of the genome of the Epstein-Barr virus (EBV). Antisera to two peptides deduced from a 1.6-kb open reading frame in the BamHI H fragment identified an 87 000-dalton nuclear polypeptide that was present in EBV-carrying cell lines that expressed the second EBV-determined nuclear antigen (EBNA-2). This polypeptide was not detected in cell lines that carried EBV variants with a deleted BamHI WYH region or in EBV-negative cell lines. Three peptides deduced from the 1.6-kb open reading frame reacted with human EBNA-positive sera, but not with EBNA-negative sera. Following affinity purification with the peptides, two of the corresponding human antibodies also reacted with the 87 000-dalton polypeptide.

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Selected References

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