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. 1985 Aug;4(8):2069–2074. doi: 10.1002/j.1460-2075.1985.tb03893.x

A yeast mutant temperature-sensitive for mitochondrial assembly is deficient in a mitochondrial protease activity that cleaves imported precursor polypeptides.

M P Yaffe, S Ohta, G Schatz
PMCID: PMC554463  PMID: 2866092

Abstract

We have previously described two yeast mutants which, at elevated temperature, stop growing and accumulate precursors to several imported mitochondrial proteins. We now show that one of these mutants (mas 1) is deficient in a matrix-located protease activity which cleaves the pre-sequences from mitochondrial precursor proteins. Isolated mas 1 mitochondria catalyze oxidative phosphorylation, exhibit respiratory control and import mitochondrial precursor polypeptides, but are defective in removing transient pre-sequences from imported precursors. The phenotype of the mas 1 mutant suggests that the matrix-located processing protease is essential for growth and for mitochondrial assembly.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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