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. 2017 Aug 4;25:18. doi: 10.1186/s40199-017-0184-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Effect of PDN-SCFE on LPS-induced activation of the JAK-STAT pathway in RAW 264.7 cells. Cells were pre-treated with PDN-SCFE for 2 h, followed by LPS stimulation for 60–240 min. Then, whole cell lystaes were prepared and used to analyze the levels of phosphorylated and non-phosphorylated form of STAT1 and STAT3 by an immune-blotting assay. Blots are representative of three independent experiments. The graphical figures represent the relative change in the ratio of phosphorylated to total protein levels. ^*** P < 0.001 vs control group; ^### P < 0.001 vs LPS-stimulated group; ^## P < 0.01 vs LPS-stimulated group; ns-non significant vs LPS-stimulated group