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. 1985 Sep;4(9):2351–2356. doi: 10.1002/j.1460-2075.1985.tb03938.x

The photocycle of the chloride pump halorhodopsin. II: Quantum yields and a kinetic model

D Oesterhelt 1, P Hegemann 1, J Tittor 1
PMCID: PMC554509  PMID: 15938054

Abstract

The quantum yield of the primary photoreaction of HR578 was determined as øgreen = 0.34±0.02 and that of the photochemical reconversion of HRL410 into HR578 as øblue = 0.01±0.004. From steady-state illumination and flash-photolysis experiments, a quantitative description of HRL410 formation and decay was made leading to a model of the photocycle in isolated, light-adapted HR. This model satisfies all known facts about HR including its photochromic behaviour.

Keywords: halorhodopsin, photocycle, light-driven chloride pump, regulation

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Selected References

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  1. Becher B., Ebrey T. G. The quantum efficiency for the photochemical conversion of the purple membrane protein. Biophys J. 1977 Feb;17(2):185–191. doi: 10.1016/S0006-3495(77)85636-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fischer U. C., Oesterhelt D. Changes in the protonation state of bacterio-opsin during reconstitution of bacteriorhodopsin. Biophys J. 1980 Jul;31(1):139–145. doi: 10.1016/S0006-3495(80)85045-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goldschmidt C. R., Ottolenghi M., Korenstein R. On the primary quantum yields in the bacteriorhodopsin photocycle. Biophys J. 1976 Jul;16(7):839–843. doi: 10.1016/S0006-3495(76)85732-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Govindjee R., Ebrey T. G., Crofts A. R. The quantum efficiency of proton pumping by the purple membrane of Halobacterium halobium. Biophys J. 1980 May;30(2):231–242. doi: 10.1016/S0006-3495(80)85091-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hazemoto N., Kamo N., Kobatake Y., Tsuda M., Terayama Y. Effect of salt on photocycle and ion-pumping of halorhodopsin and third rhodopsinlike pigment of Halobacterium halobium. Biophys J. 1984 Jun;45(6):1073–1077. doi: 10.1016/S0006-3495(84)84254-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hegemann P., Oesterbelt D., Steiner M. The photocycle of the chloride pump halorhodopsin. I: Azide-catalyzed deprotonation of the chromophore is a side reaction of photocycle intermediates inactivating the pump. EMBO J. 1985 Sep;4(9):2347–2350. doi: 10.1002/j.1460-2075.1985.tb03937.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hurley J. B., Ebrey T. G. Energy transfer in the purple membrane of Halobacterium halobium. Biophys J. 1978 Apr;22(1):49–66. doi: 10.1016/S0006-3495(78)85470-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Oesterhelt D., Hess B. Reversible photolysis of the purple complex in the purple membrane of Halobacterium halobium. Eur J Biochem. 1973 Aug 17;37(2):316–326. doi: 10.1111/j.1432-1033.1973.tb02990.x. [DOI] [PubMed] [Google Scholar]
  9. Polland H. J., Franz M. A., Zinth W., Kaiser W., Hegemann P., Oesterhelt D. Picosecond events in the photochemical cycle of the light-driven chloride-pump halorhodopsin. Biophys J. 1985 Jan;47(1):55–59. doi: 10.1016/S0006-3495(85)83876-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schobert B., Lanyi J. K., Cragoe E. J., Jr Evidence for a halide-binding site in halorhodopsin. J Biol Chem. 1983 Dec 25;258(24):15158–15164. [PubMed] [Google Scholar]
  11. Steiner M., Oesterhelt D., Ariki M., Lanyi J. K. Halide binding by the purified halorhodopsin chromoprotein. I. Effects on the chromophore. J Biol Chem. 1984 Feb 25;259(4):2179–2184. [PubMed] [Google Scholar]
  12. Steiner M., Oesterhelt D. Isolation and properties of the native chromoprotein halorhodopsin. EMBO J. 1983;2(8):1379–1385. doi: 10.1002/j.1460-2075.1983.tb01595.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stoeckenius W., Bogomolni R. A. Bacteriorhodopsin and related pigments of halobacteria. Annu Rev Biochem. 1982;51:587–616. doi: 10.1146/annurev.bi.51.070182.003103. [DOI] [PubMed] [Google Scholar]
  14. Tsuda M., Hazemoto N., Kondo M., Kamo N., Kobatake Y., Terayama Y. Two photocycles in halobacterium halobium that lacks bacteriorhodopsin. Biochem Biophys Res Commun. 1982 Oct 15;108(3):970–976. doi: 10.1016/0006-291x(82)92094-0. [DOI] [PubMed] [Google Scholar]

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