Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1985 Sep;4(9):2365–2368. doi: 10.1002/j.1460-2075.1985.tb03940.x

Mechanism of control of cytochrome oxidase activity by the electrochemical-potential gradient.

M Brunori, P Sarti, A Colosimo, G Antonini, F Malatesta, M G Jones, M T Wilson
PMCID: PMC554511  PMID: 3000774

Abstract

Cytochrome c oxidation by bovine cytochrome oxidase embedded into liposomal vesicles with high respiratory control ratio (RCR = 6-10) has been studied by rapid-mixing experiments in the presence and absence of different ionophores. Kinetic analysis of the reaction indicates a linkage between the intrinsic activity of the enzyme, the efficiency of coupling and the electrochemical potential across the membrane. A simple model, based on two allosteric states with different catalytic properties in rapid equilibrium, is presented and successfully applied in the simulation of the observed time-course.

Full text

PDF
2365

Images in this article

2366Fig. 2.
on p.2366

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexandre A., Reynafarje B., Lehninger A. L. Stoichiometry of vectorial H+ movements coupled to electron transport and to ATP synthesis in mitochondria. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5296–5300. doi: 10.1073/pnas.75.11.5296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Antonini E., Brunori M., Colosimo A., Greenwood C., Wilson M. T. Oxygen "pulsed" cytochrome c oxidase: functional properties and catalytic relevance. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3128–3132. doi: 10.1073/pnas.74.8.3128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brunori M., Giardina B., Colosimo A., Falcioni G., Gill S. J. Temperature perturbation of the allosteric equilibrium in trout hemoglobin. J Biol Chem. 1980 May 10;255(9):3841–3843. [PubMed] [Google Scholar]
  4. Errede B., Kamen M. D. Comparative kinetic studies of cytochromes c in reactions with mitochondrial cytochrome c oxidase and reductase. Biochemistry. 1978 Mar 21;17(6):1015–1027. doi: 10.1021/bi00599a012. [DOI] [PubMed] [Google Scholar]
  5. Hinkle P. C., Kim J. J., Racker E. Ion transport and respiratory control in vesicles formed from cytochrome oxidase and phospholipids. J Biol Chem. 1972 Feb 25;247(4):1338–1339. [PubMed] [Google Scholar]
  6. Hopfield J. J., Shulman R. G., Ogawa S. An allosteric model of hemoglobin. I. Kinetics. J Mol Biol. 1971 Oct 28;61(2):425–443. doi: 10.1016/0022-2836(71)90391-3. [DOI] [PubMed] [Google Scholar]
  7. Malmström B. G. Cytochrome c oxidase. Structure and catalytic activity. Biochim Biophys Acta. 1979 Dec 13;549(3-4):281–303. [PubMed] [Google Scholar]
  8. Pietrobon D., Azzone G. F., Walz D. Effect of funiculosin and antimycin A on the redox-driven H+-pumps in mitochondria: on the nature of "leaks'. Eur J Biochem. 1981 Jul;117(2):389–394. doi: 10.1111/j.1432-1033.1981.tb06350.x. [DOI] [PubMed] [Google Scholar]
  9. Proteau G., Wrigglesworth J. M., Nicholls P. Protonmotive functions of cytochrome c oxidase in reconstituted vesicles. Influence of turnover rate on 'proton translocation'. Biochem J. 1983 Jan 15;210(1):199–205. doi: 10.1042/bj2100199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Proteau G., Wrigglesworth J. M., Nicholls P. Protonmotive functions of cytochrome c oxidase in reconstituted vesicles. Influence of turnover rate on 'proton translocation'. Biochem J. 1983 Jan 15;210(1):199–205. doi: 10.1042/bj2100199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sarti P., Colosimo A., Brunori M., Wilson M. T., Antonini E. Kinetic studies on cytochrome c oxidase inserted into liposomal vesicles. Effect of ionophores. Biochem J. 1983 Jan 1;209(1):81–89. doi: 10.1042/bj2090081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sigel E., Carafoli E. The proton pump of cytochrome c oxidase and its stoichiometry. Eur J Biochem. 1978 Aug 15;89(1):119–123. doi: 10.1111/j.1432-1033.1978.tb20903.x. [DOI] [PubMed] [Google Scholar]
  13. Terada H. The interaction of highly active uncouplers with mitochondria. Biochim Biophys Acta. 1981 Dec 30;639(3-4):225–242. doi: 10.1016/0304-4173(81)90011-2. [DOI] [PubMed] [Google Scholar]
  14. Wikstrom M. K. Proton pump coupled to cytochrome c oxidase in mitochondria. Nature. 1977 Mar 17;266(5599):271–273. doi: 10.1038/266271a0. [DOI] [PubMed] [Google Scholar]
  15. Wilson M. T., Peterson J., Antonini E., Brunori M., Colosimo A., Wyman J. A plausible two-state model for cytochrome c oxidase. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7115–7118. doi: 10.1073/pnas.78.11.7115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. YONETANI T. Studies on cytochrome oxidase. III. Improved preparation and some properties. J Biol Chem. 1961 Jun;236:1680–1688. [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES