Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1985 Oct;4(10):2439–2443. doi: 10.1002/j.1460-2075.1985.tb03953.x

Sequence comparison of `states' of a1-m1 suggests a model of Spm (En) action

Zsuzsanna Schwarz-Sommer 1, Alfons Gierl 1, Rita Berndtgen 1, Heinz Saedler 1
PMCID: PMC554526  PMID: 15929215

Abstract

Two states of the a1-m1 allele featuring different phenotypes in the absence as well as in the presence of Spm or En have been cloned and sequenced. The insertion site and orientation of the Inhibitor (I) element within the two alleles is identical. The sizes of the I elements differ, being 2.2 kb in state 6078 and 789 bp in state 5719A-1. The internal deletion in state 5719A-1 affects sequences within one side of the terminal inverted repeats of the I element. This alteration can be correlated with the decreased response of this state to the Mutator function of Spm. A model for the interaction between Spm (En)-encoded functions and the receptor element is discussed explaining the phenotypic differences between the states of the locus.

Keywords: transposable element, inhibitor, S and M action, DNA sequence, Zea mays

Full text

PDF
2439

Images in this article

2442Fig. 3.
on p.2442

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dooner H. K., Nelson O. E. Controlling element-induced alterations in UDPglucose:flavonoid glucosyltransferase, the enzyme specified by the bronze locus in maize. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5623–5627. doi: 10.1073/pnas.74.12.5623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fedoroff N., Shure M., Kelly S., Johns M., Furtek D., Schiefelbein J., Nelson O. Isolation of Spm controlling elements from maize. Cold Spring Harb Symp Quant Biol. 1984;49:339–345. doi: 10.1101/sqb.1984.049.01.040. [DOI] [PubMed] [Google Scholar]
  3. Gierl A., Schwarz-Sommer Z., Saedler H. Molecular interactions between the components of the En-I transposable element system of Zea mays. EMBO J. 1985 Mar;4(3):579–583. doi: 10.1002/j.1460-2075.1985.tb03669.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nelson O. E., Klein A. S. Characterization of an spm-controlled bronze-mutable allele in maize. Genetics. 1984 Apr;106(4):769–779. doi: 10.1093/genetics/106.4.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nevers P., Saedler H. Transposable genetic elements as agents of gene instability and chromosomal rearrangements. Nature. 1977 Jul 14;268(5616):109–115. doi: 10.1038/268109a0. [DOI] [PubMed] [Google Scholar]
  6. O'Reilly C., Shepherd N. S., Pereira A., Schwarz-Sommer Z., Bertram I., Robertson D. S., Peterson P. A., Saedler H. Molecular cloning of the a1 locus of Zea mays using the transposable elements En and Mu1. EMBO J. 1985 Apr;4(4):877–882. doi: 10.1002/j.1460-2075.1985.tb03713.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Peterson P. A. Instability among the components of a regulatory element transposon in maize. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):447–455. doi: 10.1101/sqb.1981.045.01.059. [DOI] [PubMed] [Google Scholar]
  8. Saedler H., Nevers P. Transposition in plants: a molecular model. EMBO J. 1985 Mar;4(3):585–590. doi: 10.1002/j.1460-2075.1985.tb03670.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schwarz-Sommer Z., Gierl A., Cuypers H., Peterson P. A., Saedler H. Plant transposable elements generate the DNA sequence diversity needed in evolution. EMBO J. 1985 Mar;4(3):591–597. doi: 10.1002/j.1460-2075.1985.tb03671.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schwarz-Sommer Z., Gierl A., Klösgen R. B., Wienand U., Peterson P. A., Saedler H. The Spm (En) transposable element controls the excision of a 2-kb DNA insert at the wx allele of Zea mays. EMBO J. 1984 May;3(5):1021–1028. doi: 10.1002/j.1460-2075.1984.tb01922.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shure M., Wessler S., Fedoroff N. Molecular identification and isolation of the Waxy locus in maize. Cell. 1983 Nov;35(1):225–233. doi: 10.1016/0092-8674(83)90225-8. [DOI] [PubMed] [Google Scholar]
  12. Tuschall D. M., Hannah L. C. Altered Maize Endosperm Adp-Glucose Pyrophosphorylases from Revertants of a SHRUNKEN-2-DISSOCIATION ALLELE. Genetics. 1982 Jan;100(1):105–111. doi: 10.1093/genetics/100.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES