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. 1985 Oct;4(10):2457–2460. doi: 10.1002/j.1460-2075.1985.tb03956.x

Assembly of the mannose-6-phosphate receptor into reconstituted clathrin coats.

B M Pearse
PMCID: PMC554529  PMID: 2865131

Abstract

In ionic conditions in which clathrin coats are stable, the mannose-6-phosphate receptor associates with the 100-kd/50-kd coat complexes purified from bullock brain coated vesicles. These aggregates exist as striking spherical structures of 300-1000 A diameter. When clathrin is included in the assembly mixture, cages are formed which apparently encapsulate these aggregates, giving, in the absence of lipid, structures resembling full coated vesicles.

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