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. 1985 Oct;4(10):2553–2560. doi: 10.1002/j.1460-2075.1985.tb03970.x

Bloom syndrome B-lymphoblastoid cells are hypersensitive towards carcinogen and tumor promoter-induced chromosomal alterations and growth in agar.

Y Shiraishi
PMCID: PMC554543  PMID: 3876929

Abstract

The effects of the carcinogens (4NQO, 4-nitroquinoline-N-oxide; MNNG, N-methyl-N'-nitro-N-nitrosoguanidine; AFLG1, aflatoxin G1; AFLB1, aflatoxin B1; BNU, butylnitrosourea; MNU, methylnitrosourea) and the tumor promoter (TPA, 12-O-tetradecanoylphorbol-13-acetate) on sister chromatid exchanges (SCE), chromosome aberrations and colony formation (CF) were examined in three types of Bloom syndrome (BS) B-lymphoblastoid cell lines (B-LCLs); type I with normal SCE and normal karyotype; type II with high SCE and normal karyotypes; type III with high SCE and abnormal karyotypes. BS type I cells had the same SCE and CF response as normal cells to these carcinogens and TPA. In BS type II and III cells treated with carcinogens the SCE frequency increased to 140/cell from a baseline of 70/cell versus an increase of only 10/cell in normal cells. Colony formation occurred at the concentrations that caused the highest SCE. TPA caused a significant SCE increase and highly enhanced CF with dose dependency only in type III cells, suggesting that type III cells may be already in a pre-malignant state; type II cells appear to be one step behind those of type III in the process of becoming malignant. BS type II and III cells may be usable to establish a sensitive system to detect SCE-inducing agents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chaganti R. S., Schonberg S., German J. A manyfold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4508–4512. doi: 10.1073/pnas.71.11.4508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Evans C. H., DiPaolo J. A. Neoplastic transformation of guinea pig fetal cells in culture induced by chemical carcinogens. Cancer Res. 1975 Apr;35(4):1035–1044. [PubMed] [Google Scholar]
  3. Fujiwara Y., Kano Y., Tatsumi M., Paul P. Effects of a tumor promoter and an anti-promoter on spontaneous and UV-induced 6-thioguanine-resistant mutations and sister-chromatid exchanges in V79 Chinese hamster cells. Mutat Res. 1980 Jul;71(2):243–251. doi: 10.1016/0027-5107(80)90076-7. [DOI] [PubMed] [Google Scholar]
  4. German J., Bloom D., Passarge E. Bloom's syndrome XI. Progress report for 1983. Clin Genet. 1984 Feb;25(2):166–174. doi: 10.1111/j.1399-0004.1984.tb00480.x. [DOI] [PubMed] [Google Scholar]
  5. Kinsella A. R., Radman M. Tumor promoter induces sister chromatid exchanges: relevance to mechanisms of carcinogenesis. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6149–6153. doi: 10.1073/pnas.75.12.6149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Klein G. Lymphoma development in mice and humans: diversity of initiation is followed by convergent cytogenetic evolution. Proc Natl Acad Sci U S A. 1979 May;76(5):2442–2446. doi: 10.1073/pnas.76.5.2442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Krepinsky A. B., Heddle J. A., German J. Sensitivity of Bloom's syndrome lymphocytes to ethyl methanesulfonate. Hum Genet. 1979;50(2):151–156. doi: 10.1007/BF00390236. [DOI] [PubMed] [Google Scholar]
  8. Loveday K. S., Latt S. A. The effect of a tumor promotor, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), on sister-chromatid exchange formation in cultured Chinese hamster cells. Mutat Res. 1979 Aug;67(4):343–348. doi: 10.1016/0165-1218(79)90030-2. [DOI] [PubMed] [Google Scholar]
  9. Perry P., Wolff S. New Giemsa method for the differential staining of sister chromatids. Nature. 1974 Sep 13;251(5471):156–158. doi: 10.1038/251156a0. [DOI] [PubMed] [Google Scholar]
  10. Shiraishi Y., Freeman A. I., Sandberg A. A. Increased sister chromatid exchange in bone marrow and blood cells from Bloom's syndrome. Cytogenet Cell Genet. 1976;17(4):162–173. doi: 10.1159/000130710. [DOI] [PubMed] [Google Scholar]
  11. Shiraishi Y., Kubonishi I., Sandberg A. A. Establishment of B-lymphoid cell lines retaining cytogenetic characteristics of Bloom syndrome. Cancer Genet Cytogenet. 1983 Jun;9(2):129–138. doi: 10.1016/0165-4608(83)90033-x. [DOI] [PubMed] [Google Scholar]
  12. Shiraishi Y., Sandberg A. A. Effects of chemicals on the frequency of sister chromatid exchanges and chromosome aberrations in normal and Bloom's syndrome lymphocytes. Cytobios. 1979;26(102):91–108. [PubMed] [Google Scholar]
  13. Shiraishi Y., Yoshimoto S., Miyoshi I., Kondo N., Orii T., Sandberg A. A. Dimorphism of sister chromatid exchange in Bloom's syndrome B- and T-cell lines transformed with Epstein-Barr and adult T-cell leukemia viruses. Cancer Res. 1983 Aug;43(8):3836–3840. [PubMed] [Google Scholar]
  14. Shiraishi Y., Yosida T. H., Sandberg A. A. Analyses of bromodeoxyuridine-associated sister chromatid exchanges (SCEs) in Bloom syndrome based on cell fusion: single and twin SCEs in endoreduplication. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4369–4373. doi: 10.1073/pnas.80.14.4369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thompson L. H., Baker R. M., Carrano A. V., Brookman K. W. Failure of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate to enhance sister chromatid exchange, mitotic segregation, or expression of mutations in Chinese hamster cells. Cancer Res. 1980 Sep;40(9):3245–3251. [PubMed] [Google Scholar]
  16. Zech L., Haglund U., Nilsson K., Klein G. Characteristic chromosomal abnormalities in biopsies and lymphoid-cell lines from patients with Burkitt and non-Burkitt lymphomas. Int J Cancer. 1976 Jan 15;17(1):47–56. doi: 10.1002/ijc.2910170108. [DOI] [PubMed] [Google Scholar]

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