Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1985 Dec 1;4(12):3183–3188. doi: 10.1002/j.1460-2075.1985.tb04063.x

Mapping of the c-myc, pvt-1 and immunoglobulin kappa genes in relation to the mouse plasmacytoma-associated variant (6;15) translocation breakpoint.

M Banerjee, F Wiener, J Spira, M Babonits, M G Nilsson, J Sumegi, G Klein
PMCID: PMC554640  PMID: 3937724

Abstract

A variant mouse plasmacytoma (MPC)-associated translocation chromosome has arisen by pericentric inversion and exchange of the distal segments of a Robertsonian 6;15 fusion chromosome in the CAK TEPC 1198 mouse plasmacytoma, as described earlier. In situ hybridization was performed on the normal and the inverted Rb chromosomes, using myc and kappa probes. On the normal Rb chromosome, myc was in the 15 D2/3 region, whereas kappa hybridized in the 6 C2 area, as expected. On the inverted Rb chromosome, myc remains on the centrometric side of the translocation breakpoint on the chromosome 15-derived portion, whereas kappa has moved to the chromosome 6-derived segment that joined the same breakpoint on the telomeric side. Taken together with our recent demonstration that the murine c-myc locus is oriented 'head up' on chromosome 15, and with the results of Cory and co-workers concerning the relationship between the kappa gene and the associated pvt-1 region in the CAK TEPC 1198 tumor, the following conclusions can be drawn: (i) in the variant translocation of the CAK TEPC 1198 MPC, the breakage occurs 3' of the c-myc gene, as in the human Burkitt lymphoma-associated variant translocations; (ii) the pvt-1 gene on chromosome 15 is distal to the myc gene; (iii) the kappa light chain locus is oriented 'head up' on mouse chromosome 6 and faces pvt-1 and, beyond it, c-myc, in a head-to-tail configuration.

Full text

PDF
3183

Images in this article

3184Fig. 1.
on p.3184
3185Fig. 2.
on p.3185
3185Fig. 3.
on p.3185
3186Fig. 5.
on p.3186
3187Fig. 6.
on p.3187

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams J. M., Gerondakis S., Webb E., Corcoran L. M., Cory S. Cellular myc oncogene is altered by chromosome translocation to an immunoglobulin locus in murine plasmacytomas and is rearranged similarly in human Burkitt lymphomas. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1982–1986. doi: 10.1073/pnas.80.7.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Breiner A. V., Brandt C. R., Milcarek C., Sweet R. W., Ziv E., Burstein Y., Schechter I. Somatic DNA rearrangement generates functional rat immunoglobulin kappa chain genes: the J kappa gene cluster is longer in rat than in mouse. Gene. 1982 May;18(2):165–174. doi: 10.1016/0378-1119(82)90114-7. [DOI] [PubMed] [Google Scholar]
  3. Cory S., Graham M., Webb E., Corcoran L., Adams J. M. Variant (6;15) translocations in murine plasmacytomas involve a chromosome 15 locus at least 72 kb from the c-myc oncogene. EMBO J. 1985 Mar;4(3):675–681. doi: 10.1002/j.1460-2075.1985.tb03682.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Croce C. M., Thierfelder W., Erikson J., Nishikura K., Finan J., Lenoir G. M., Nowell P. C. Transcriptional activation of an unrearranged and untranslocated c-myc oncogene by translocation of a C lambda locus in Burkitt. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6922–6926. doi: 10.1073/pnas.80.22.6922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Erikson J., Nishikura K., ar-Rushdi A., Finan J., Emanuel B., Lenoir G., Nowell P. C., Croce C. M. Translocation of an immunoglobulin kappa locus to a region 3' of an unrearranged c-myc oncogene enhances c-myc transcription. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7581–7585. doi: 10.1073/pnas.80.24.7581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Klein G., Klein E. Oncogene activation and tumor progression. Carcinogenesis. 1984 Apr;5(4):429–435. doi: 10.1093/carcin/5.4.429. [DOI] [PubMed] [Google Scholar]
  7. Neel B. G., Jhanwar S. C., Chaganti R. S., Hayward W. S. Two human c-onc genes are located on the long arm of chromosome 8. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7842–7846. doi: 10.1073/pnas.79.24.7842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nishikura K., ar-Rushdi A., Erikson J., Watt R., Rovera G., Croce C. M. Differential expression of the normal and of the translocated human c-myc oncogenes in B cells. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4822–4826. doi: 10.1073/pnas.80.15.4822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ohno S., Babonits M., Wiener F., Spira J., Klein G., Potter M. Nonrandom chromosome changes involving the Ig gene-carrying chromosomes 12 and 6 in pristane-induced mouse plasmacytomas. Cell. 1979 Dec;18(4):1001–1007. doi: 10.1016/0092-8674(79)90212-5. [DOI] [PubMed] [Google Scholar]
  10. Ohno S., Migita S., Wiener F., Babonits M., Klein G., Mushinski J. F., Potter M. Chromosomal translocations activating myc sequences and transduction of v-abl are critical events in the rapid induction of plasmacytomas by pristane and abelson virus. J Exp Med. 1984 Jun 1;159(6):1762–1777. doi: 10.1084/jem.159.6.1762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rappold G. A., Hameister H., Cremer T., Adolph S., Henglein B., Freese U. K., Lenoire G. M., Bornkamm G. W. c-myc and immunoglobulin kappa light chain constant genes are on the 8q+ chromosome of three Burkitt lymphoma lines with t(2;8) translocations. EMBO J. 1984 Dec 1;3(12):2951–2955. doi: 10.1002/j.1460-2075.1984.tb02239.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shen-Ong G. L., Keath E. J., Piccoli S. P., Cole M. D. Novel myc oncogene RNA from abortive immunoglobulin-gene recombination in mouse plasmacytomas. Cell. 1982 Dec;31(2 Pt 1):443–452. doi: 10.1016/0092-8674(82)90137-4. [DOI] [PubMed] [Google Scholar]
  13. Stanton L. W., Watt R., Marcu K. B. Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas. Nature. 1983 Jun 2;303(5916):401–406. doi: 10.1038/303401a0. [DOI] [PubMed] [Google Scholar]
  14. Webb E., Adams J. M., Cory S. Variant (6 ; 15) translocation in a murine plasmacytoma occurs near an immunoglobulin kappa gene but far from the myc oncogene. Nature. 1984 Dec 20;312(5996):777–779. doi: 10.1038/312777a0. [DOI] [PubMed] [Google Scholar]
  15. Wiener F., Babonits M., Bregula U., Klein G., Léonard A., Wax J. S., Potter M. High resolution banding analysis of the involvement of strain BALB/c- and AKR-derived chromosomes No. 15 in plasmacytoma-specific translocations. J Exp Med. 1984 Jan 1;159(1):276–291. doi: 10.1084/jem.159.1.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wiener F. Chromosomal aberrations in murine plasmacytomas. Curr Top Microbiol Immunol. 1984;113:178–182. doi: 10.1007/978-3-642-69860-6_30. [DOI] [PubMed] [Google Scholar]
  17. Wiener F., Ohno S., Babonits M., Sümegi J., Wirschubsky Z., Klein G., Mushinski J. F., Potter M. Hemizygous interstitial deletion of chromosome 15 (band D) in three translocation-negative murine plasmacytomas. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1159–1163. doi: 10.1073/pnas.81.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wirschubsky Z., Wiener F., Bregula U., Klein G. Further studies on the asymmetry of chromosome 15 duplication in trisomic leukemias of heterozygous origin: preferential status of the AKR chromosome. Int J Cancer. 1984 Aug 15;34(2):249–254. doi: 10.1002/ijc.2910340217. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES