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. 2017 Apr 14;8(27):43602–43616. doi: 10.18632/oncotarget.17115

Figure 3. SK2 inhibition synergises with bortezomib.

Figure 3

A. LP-1 cells were cultured with the indicated concentrations of K145 with and without 6 nM bortezomib for 24 h and cell viability measured by flow cytometry using Annexin-V/PI staining. Predicted additive effects, calculated by the fractional product method [19], are shown by the dashed lines whilst actual observed combinational effects by the red lines. B. LP-1 cells were cultured with the indicated concentrations of bortezomib with and without 4 μM K145 for 24 h and cell viability measured by flow cytometry using Annexin-V/PI staining. C. 5TGM1 cells were cultured with the indicated concentrations of K145 with and without 4 nM bortezomib for 24 h and cell viability measured by flow cytometry using Annexin-V/PI staining. D. LP-1 cells were cultured with the indicated concentrations of ABC294640 with and without 6 nM bortezomib for 24 h and cell viability measured by flow cytometry using Annexin-V/PI staining. Predicted additive and observed combinational effects are shown by the dashed and red lines, respectively. Mean±SD of duplicate measurements shown. Data are representative of three independent experiments.