Figure 5. The effects of RSV on cells under GRP78 siRNA pre-treated condition.

(A) GRP78 silencing was measured by qRT-PCR and western blot. (B) The effects of RSV on cell viability in stressed HepG2 cells under GRP78 siRNA and non-targeting (NT) siRNA conditions. (C) Apoptosis analysis of the cells by the annexin V (FITC)/PI binding assay and the percentage of apoptotic cells was calculated (D). The mRNA experession of GRP78 (E) and caspase-3 (F) expression was determined by qRT-PCR. The data were presented as the mean ± sd of three independent experiments. NS= no significant difference, ^*p < 0.01, ^*p < 0.01,^***p < 0.001 vs the corresponding group.