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. 1985 Dec 1;4(12):3235–3240. doi: 10.1002/j.1460-2075.1985.tb04071.x

The abnormal location of cytoplasmic SV40 large T is not caused by failure to bind to DNA or to p53.

E Paucha, D Kalderon, W D Richardson, R W Harvey, A E Smith
PMCID: PMC554648  PMID: 3004945

Abstract

We have examined the large T encoded by an SV40 mutant, d10, which fails to localize to the nucleus. The DNA sequence of the mutant predicts the alteration of Lys 128----Thr within the sequence 127 Lys Lys Lys Arg Lys 131 of large T. The results show that d10 large T is capable of binding to SV40 DNA, to cellular DNA and to the cellular phosphoprotein p53 as well as wild-type large T. These data suggest that the cytoplasmic location of d10 large T is not due to an inability of the protein to be retained within the nucleus, but argues instead that the protein fails to reach the nucleus because it contains a defective nuclear location signal.

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