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. 1985 Dec 16;4(13A):3553–3556. doi: 10.1002/j.1460-2075.1985.tb04116.x

Cloning of opal suppressor tRNA genes of a filamentous fungus reveals two tRNASerUGA genes with unexpected structural differences.

R Debuchy, Y Brygoo
PMCID: PMC554696  PMID: 3937728

Abstract

The informational suppressors su4-1 and su8-1 of Podospora anserina were isolated by transformation of Schizosaccharomyces pombe UGA mutants. The DNA sequence revealed that they were opal (UGA) suppressor tRNAs. Wild-type alleles were also isolated by hybridization. The DNA sequence showed that they both encode species of tRNASerUGA. The gene SU8 has an 18-bp intervening sequence and its primary sequence is very different from that of SU4.

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Selected References

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