Skip to main content
. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Methods. 2017 May 18;125:45–54. doi: 10.1016/j.ymeth.2017.05.011

Figure 5.

Figure 5

U2 snRNA dynamics analyzed by smFRET by Rodgers et al. [20]. (A) Two mutually exclusive RNA structures can be adopted U2 stem II and are described by different FRET states. The smFRET reporter RNA was labeled with Cy3 (green) and Cy5 (red). (B) Histograms plotting EFRET for the smFRET reporter RNA in response to Mg2+ (C) TODPs of EFRET for stem II molecular transitions. The structural transitions of the RNA increased and changed in EFRETin the presence of Mg2+ The figure shown was adapted from reference [20] and used with permission.