Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1985 Dec 16;4(13A):3599–3604. doi: 10.1002/j.1460-2075.1985.tb04123.x

Control of gene expression in P2-related coliphages: the in vitro transcription pattern of coliphage 186.

M Pritchard, J B Egan
PMCID: PMC554703  PMID: 3912173

Abstract

Transcription in vitro of coliphage 186 DNA generated four transcripts. The most abundant transcript was that of the late control gene B and an equivalent transcript was identified for the closely related phage P2. A second transcript was from the rightward promoter at 75% and predicted to be under CI repressor control. The remaining two transcripts initiated from the one promoter located at 95% and are apparently under LexA control in vivo. The significance of these transcripts is discussed in relation to coliphage 186.

Full text

PDF
3599

Images in this article

3599Fig. 1.
on p.3599
3600Fig. 2.
on p.3600
3601Fig. 3.
on p.3601
3601Fig. 4.
on p.3601

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey J. M., Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. doi: 10.1016/s0003-2697(76)80049-8. [DOI] [PubMed] [Google Scholar]
  2. Finnegan J., Egan J. B. In vivo transcription studies of coliphage 186. J Virol. 1981 Jun;38(3):987–995. doi: 10.1128/jvi.38.3.987-995.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Finnegan J., Egan J. B. Physical map of the coliphage 186 chromosome. I. Gene content of the BamHI, PstI and other restriction fragments. Mol Gen Genet. 1979;172(3):287–293. doi: 10.1007/BF00271728. [DOI] [PubMed] [Google Scholar]
  4. Gould A. R., Symons R. H. Cucumber mosaic virus RNA 3. Determination of the nucleotide sequence provides the amino acid sequences of protein 3A and viral coat protein. Eur J Biochem. 1982 Aug;126(2):217–226. [PubMed] [Google Scholar]
  5. Haseloff J., Symons R. H. Chrysanthemum stunt viroid: primary sequence and secondary structure. Nucleic Acids Res. 1981 Jun 25;9(12):2741–2752. doi: 10.1093/nar/9.12.2741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hocking S. M., Egan J. B. Genetic map of coliphage 186 from a novel use of marker rescue frequencies. Mol Gen Genet. 1982;187(1):87–95. doi: 10.1007/BF00384388. [DOI] [PubMed] [Google Scholar]
  7. Hocking S. M., Egan J. B. Genetic studies of coliphage 186. I. Genes associated with phage morphogenesis. J Virol. 1982 Dec;44(3):1056–1067. doi: 10.1128/jvi.44.3.1056-1067.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hocking S. M., Egan J. B. Genetic studies of coliphage 186. II. Genes associated with phage replication and host cell lysis. J Virol. 1982 Dec;44(3):1068–1071. doi: 10.1128/jvi.44.3.1068-1071.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kafatos F. C., Jones C. W., Efstratiadis A. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 1979 Nov 24;7(6):1541–1552. doi: 10.1093/nar/7.6.1541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kalionis B., Egan J. B. Orientation of separated DNA strands of coliphage 186 relative to its genetic map. Gene. 1981 Oct;15(1):95–98. doi: 10.1016/0378-1119(81)90107-4. [DOI] [PubMed] [Google Scholar]
  11. Lehrach H., Diamond D., Wozney J. M., Boedtker H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977 Oct 18;16(21):4743–4751. doi: 10.1021/bi00640a033. [DOI] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  13. Sprizhitsky YuA, Kopylov V. M. The SOS system of Escherichia coli in the regulation of bacteriophage lambda development. FEBS Lett. 1983 Aug 22;160(1-2):7–10. doi: 10.1016/0014-5793(83)80925-9. [DOI] [PubMed] [Google Scholar]
  14. Sunshine M. G. Dependence of education on P2 int product. Virology. 1972 Jan;47(1):61–67. doi: 10.1016/0042-6822(72)90239-5. [DOI] [PubMed] [Google Scholar]
  15. Sunshine M. G., Sauer B. A bacterial mutation blocking P2 phage late gene expression. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2770–2774. doi: 10.1073/pnas.72.7.2770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Walker G. C. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev. 1984 Mar;48(1):60–93. doi: 10.1128/mr.48.1.60-93.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Westö A., Ljungquist E. A restriction endonuclease cleavage map of bacteriophage P2. Mol Gen Genet. 1979 Mar 9;171(1):91–102. doi: 10.1007/BF00274019. [DOI] [PubMed] [Google Scholar]
  19. Woods W. H., Egan J. B. Prophage induction of noninducible coliphage 186. J Virol. 1974 Dec;14(6):1349–1356. doi: 10.1128/jvi.14.6.1349-1356.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES