Abstract
Objective
Nogo-A is an axon regeneration inhibitor, and its function in central nervous system (CNS) is still unknown. The present study is to explore the relationship between the expression of Nogo-A and the malignancy of oligodendroglial tumors in patients.
Methods
Tumor tissue samples with different malignancy grade were obtained from the hospitals. The samples used for detection had been diagnosed as oligodendroglial tumors (oligodendroglioma or anaplastic oligodendroglioma). The expression of Nogo-A was detected by immunohistochemistry and western-blot analysis. The correlation test between the Nogo-A expression and the morphological changes (the percentages of atypical cells and mitotic cells in the tumors) related to the malignancy of tumor tissues was performed.
Results
There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligodendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group.
Conclusion
Nogo-A expression was negatively correlated with the malignancy grade of oligodendroglial tumors.
Keywords: Nogo-A, oligodendroglial tumors, immunohistochemistry
摘要
目的
探讨 Nogo-A 蛋白在少突胶质细胞肿瘤中的表达水平与该肿瘤恶性程度之间的关系。
方法
选取来自临床上经病理检查确诊为少突胶质细胞肿瘤的组织标本, 其中少突胶质细胞瘤 16 例, 间变型少突胶质细胞瘤 10 例。 免疫组织化学染色检测 Nogo-A 的表达, 并与同视野下瘤细胞的核分裂与核异型性比例作相关分析; 另外, 把病理标本按照分级进行蛋白印迹 试验。
结果
免疫组织化学结果显示, Nogo-A 免疫阳性染色的光密度值与肿瘤细胞核分裂、 核异型比例呈明显的负相关, 对蛋白印迹试验结果进行光密度分析也发现少突胶质细胞瘤中的 Nogo-A 蛋白条带灰度值明显高于间变型少突胶质细胞瘤中的灰度值。
结论
Nogo-A 蛋白在少突胶质细胞肿瘤中的表达水平与该肿瘤恶性程度呈负相关。
关键词: Nogo-A, 少突胶质细胞肿瘤, 免疫组织化学
Reference
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