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. 2008 Feb 7;23(5):263–270. doi: 10.1007/s12264-007-0039-5

Targeted migration and differentiation of engrafted neural precursor cells in amyloid β-treated hippocampus in rats

胚胎干细胞来源的神经前体细胞移植 β-淀粉样蛋白损伤大鼠海马后的靶向迁移与分化

Jun Tang 1, Hai-Wei Xu 1,✉, Xiao-Tang Fan 2, Zhi-Fang Li 1,3, Da-Bing Li 1, Li Yang 1, Guang-Ji Zhou 1
PMCID: PMC5550573  PMID: 17952134

Abstract

Objective

To observe the migration and differentiation of the neural precursor cells (NPCs) that derived from murine embryonic stem cells (ESCs) when they were transplanted into amyloid β (Aβ)-treated rat hippocampus.

Methods

MESPU35, a murine ESC cell line that express the enhanced green fluorescent protein (EGFP), was induced differentiation into nestin-positive NPCs by modified serum-free methods. The Aβ plaques and the differentiation of the grafted cells were observed by immunofluorescent staining.

Results

Comparing 16 weeks with 4 weeks post-transplantation, the migration distance increased about 5 times; the rate of migratory NPCs differentiating into glial fibrillary acidic protein (GFAP)-positive cells kept rising from (30.41±1.45)% to (49.25±1.23)%, and the rate of NPCs differentiating into neurofilament 200 (NF200) positive cells increased from (16.68±0.95)% to (27.94±1.21)%. Meanwhile, the GFAP-positive cells targeting to the ipsilateral side of Aβ plaques increased from 60.2% to 81.3%, while the NF200-positive cells increased from 61.3% to 84.1%. The migration distance had significant positive linear correlations to the neuronal differentiation rate (r = 0.991) and to the astrocytic differentiation rate (r = 0.953).

Conclusion

Engrafted NPCs migrate targetedly to the Aβ injection site and differentiate into neurons and astrocytes.

Keywords: embryonic stem cells, amyloid β peptide, cell transplantation, differentiation, migration, rat

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