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. 2008 Feb 7;23(5):293–299. doi: 10.1007/s12264-007-0044-8

Expression of three distinct families of calcium-activated chloride channel genes in the mouse dorsal root ganglion

小鼠背根神经节中三个不同的钙激活氯离子通道基因家族的表达

Mohammed Al-Jumaily 1,✉, Alexei Kozlenkov 1, Ilana Mechaly 1,2, Agnes Fichard 1,2, Valerie Matha 1, Frederique Scamps 1, Jean Valmier 1,2, Patrick Carroll 1
PMCID: PMC5550578  PMID: 17952139

Abstract

Objective

A calcium-activated chloride current (I Cl(Ca)) has been observed in medium-sized sensory neurons of the dorsal root ganglion (DRG). Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule(s) underlying this current.

Methods

Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have I Cl(Ca) current inducing properties.

Results

We showed the detection of transcripts representing several members of these families, i.e. chloride channel calcium-activated (CLCA), Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury.

Conclusion

Our analysis revealed that that mBest1 and Tweety2 appear as the best candidates to play a role in the injury-induced I Cl(Ca) in DRG neurons.

Keywords: chloride channel calcium activated, bestrophin, tweety, chloride channel, dorsal root ganglion

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