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. 1983;2(4):543–548. doi: 10.1002/j.1460-2075.1983.tb01460.x

Poly(ADP-ribose) polymerase auto-modification and interaction with DNA: electron microscopic visualization.

G de Murcia, J Jongstra-Bilen, M E Ittel, P Mandel, E Delain
PMCID: PMC555058  PMID: 6313345

Abstract

The interaction between purified calf thymus poly(ADP-ribose) polymerase and its activating co-purified DNA (sDNA) was investigated by electron microscopy. We have shown that the enzyme-DNA complex possesses a nucleosome-like structure. The enzyme-bound DNA (sDNA) was found to be enriched in single-stranded regions and branched structures, presumed to be replication forks. The auto-ribosylated polymerase as well as the branched poly(ADP-ribose) formed were visualized by dark field electron microscopy during the auto-ADP-ribosylation reaction and the possible mechanism of this phenomenon is discussed.

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Selected References

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