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. 2008 Feb 1;23(4):209–214. doi: 10.1007/s12264-007-0031-0

Repair of glutamate-induced excitotoxic neuronal damage mediated by intracerebroventricular transplantation of neural stem cells in adult mice

脑室内神经干细胞移植修复谷氨酸导致的成年小鼠神经损伤

Juan Ma 1, Li-Jian Yu 1,✉, Run-Di Ma 1, Yong-Ping Zhang 1, Juan-Zhi Fang 1, Xiao-Yu Zhang 1, Ting-Xi Yu 1,✉
PMCID: PMC5550583  PMID: 17687395

Abstract

Objective

To investigate a possibility of repairing damaged brain by intracerebroventricular transplantation of neural stem cells (NSCs) in the adult mice subjected to glutamate-induced excitotoxic injury.

Methods

Mouse NSCs were isolated from the brains of embryos at 15-day postcoitum (dpc). The expression of nestin, a special antigen for NSC, was detected by immunocytochemistry. Immunofluorescence staining was carried out to observe the survival and location of transplanted NSCs. The animals in the MSG+NSCs group received intracerebroventricular transplantation of NSCs (approximately 1.0×105 cells) separately on day 1 and day 10 after 10-d MSG exposure (4.0 g/kg per day). The mice in control and MSG groups received intracerebroventricular injection of Dulbecco’s minimum essential medium (DMEM) instead of NSCs. On day 11 after the last NSC transplantation, the test of Y-maze discrimination learning was performed, and then the histopathology of the animal brains was studied to analyze the MSG-induced functional and morphological changes of brain and the effects of intracerebroventricular transplantation of NSCs on the brain repair.

Results

The isolated cells were Nestin-positive. The grafted NSCs in the host brain were region-specifically survived at 10-d post-transplantation. Intracerebroventricular transplantation of NSCs obviously facilitated the brain recovery from glutamate-induced behavioral disturbances and histopathological impairs in adult mice.

Conclusion

Intracerebroventricular transplantation of NSCs may be feasible in repairing diseased or damaged brain tissue.

Keywords: brain repair, neural stem cells, transplantation, excitotoxic injury, mice

Contributor Information

Li-Jian Yu, Phone: 86-759-2382041, FAX: 86-759-2382424, Email: ywyj9578@sohu.com.

Ting-Xi Yu, Email: yutingxi@yahoo.com.

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