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. 2008 Feb 1;23(4):221–228. doi: 10.1007/s12264-007-0033-y

Involvement of MAPK/ERK kinase-ERK pathway in exogenous bFGF-induced Egr-1 binding activity enhancement in anoxia-reoxygenation injured astrocytes

MAPK/ERK 激酶-ERK 信号通路参与外源性 bFGF 对缺氧-复氧损伤后星形胶质细胞内 Egr-1 结合活性的调节

Ying Liu 1, Jin-Biao Lu 1, Qi Chen 1, Zhu-Rong Ye 1,✉
PMCID: PMC5550585  PMID: 17687397

Abstract

Objective

Intravenous administration of basic fibroblast growth factor (bFGF) is effective to reduce the volume of cerebral infract due to ischemia. This study was designed to investigate the molecular mechanism, especially the signal transduction pathways, involved in this protective role of bFGF.

Methods

Anoxia-reoxygenation treated atrocytes were used to study the role of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MAPK/ERK kinase, MEK)-ERK signaling pathway after exogenous bFGF administration by Western blot. Electrophoretic mobile shift assay was used to detect the binding activity of early growth response factor-1 (Egr-1), an important transcription factor for endogenous bFGF.

Results

BFGF could protect some signal transduction proteins from the oxygen-derived free radicals induced degradation. ERK1/2 was activated and involved in Egr-1 binding activity enhancement induced by exogenous bFGF.

Conclusion

MEK-ERK MAPK cascade may be an important signal transduction pathway contributed to bFGF induced enhancement of Egr-1 binding activity in anoxia-reoxygenation injured astrocytes.

Keywords: extracellular signal-regulated kinase, mitogen-activated protein kinase, free radicals, fibroblast growth factor 2, early growth response protein 1, astrocyte

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