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. 2008 Feb 1;23(4):229–235. doi: 10.1007/s12264-007-0034-x

Possible involvement of integrin signaling pathway in the process of recovery from restraint stress in rats

整合素信号通路可能参与大鼠束缚应激后的恢复过程

Yu-Zhen Gao 1,2,3, Shi-Yu Guo 1,2,3, Qi-Zhang Yin 1,2, Xiang-Qin Cui 4, Tadashi Hisamitsu 3, Xing-Hong Jiang 1,2,✉
PMCID: PMC5550586  PMID: 17687398

Abstract

Objective

To search novel genes or pathways involved in the recovery process after restraint stress in rats.

Methods

We compared the hypothalamus transcriptional profiles of two different recovery patterns (fast recovery vs slow recovery) from restraint stress in rats using oligonucleotide microarray, the recovery pattern was determined by the decrement of plasma adrenocorticotropic-hormone (ACTH) and corticosterone levels during one hour recovery period after stress. A real-time quantitative RT-PCR was applied to validate the differential expressed genes.

Results

Analysis of the microarray data showed that most of genes were not differentially expressed between fast recovery group and slow recovery group. Among the differentially expressed genes we found that talin, together with serine/threonine protein phosphatase PP1-beta catalytic subunit (PP-1B) and integrin α-6 precursor (VLA-6) genes, were at least 1.5 fold up-regulated in the fast recovery group, while junctional adhesion molecule 1 (F11r) was 1.5 fold down-regulated in the fast recovery group.

Conclusion

The results implied that integrin signaling pathway may be involved in the recovery from restraint stress in rats. The present study provided a global overview of hypothalamus transcriptional profiles during the process of recovery from the restraint stress in rats. The integrin signaling pathway seems to be involved in the recovery process, which deserves further study to clarify the integrin-mediated recovery mechanism after restraint stress.

Keywords: physical restraint, adrenocorticotropic hormone, corticosterone, post-stress recovery, oligonucleotide microarray

Contributor Information

Tadashi Hisamitsu, Phone: 81-3-37848110, FAX: 81-3-37845368, Email: tadashi@med.showa-u.ac.jp.

Xing-Hong Jiang, Phone: 86-512-65880126, Email: jiangxinghong@suda.edu.cn.

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