Abstract
Objective
To study the expression change of interleukin-8 (IL-8) gene in the basilar artery of rabbit and the effect of IL-8 on the development of cerebral vasospasm induced by experimental subarachnoid hemorrhage (SAH).
Methods
Thirty five healthy Japanese White Rabbits were randomly divided into saline-control group and experimental group. The experimental group was subdivided into four groups, representing day 1, 4, 7 and 14 after the first blood injection of SAH. The delayed cerebral vasospasm (DCVS) model was established by double injection of autologous blood into the cisterna magna. The expression change of cytokine IL-8 mRNA in the basilar artery was analyzed by RTPCR.
Results
The expression of IL-8 gene increased on day 4–7 after the first blood injection of SAH compared with control (P < 0.001), and decreased to normal on day 14. The expression of IL-8 gene in the SAH groups were positively correlated with the degree of basilar artery stenosis (r = 0.642, P < 0.01).
Conclusion
The expression level of IL-8 gene in basilar arteries was intimately associated with the degree of cerebral vasospasm, suggesting that IL-8 may play an important role in the DCVS after SAH as an immunological inflammatory factor.
Keywords: intracranial vasospasm, interleukin-8, RT-PCR, subarachnoid hemorrhage
摘要
目的
探讨实验性蛛网膜下腔出血(SAH)诱发脑血맜痉挛时, 白细胞介素-8(IL-8)基因在兔脑基底动脉中表达的变化及在诱发脑血맜痉挛中的作用。
方法
35只健康日本大耳白兔随机分为生理盐水组、 SAH组。 SAH 组根据第一次注血时间又分为四组, 分别为第一次注血后第1、 4、 7、 14天。 以枕大池二次注血法构建迟发性脑血맜痉挛模型, 采用 RT-PCR 法观察兔基底动脉中细胞因子 IL-8 mRNA 表达的变化。
结果
IL-8mRNA在 SAH 组第一次注血后第4–7 天升高, 14天趋于正常。 SAH 组 IL-8 的表达水平与基底动脉的狭窄程度呈正相关(r = 0.642, P < 0.01)。
结论
IL-8 在基底动脉中的表达水平与脑血맜痉挛的程度紧密相关, 提示 IL-8 可能作为免疫/炎症因素因素参与了 SAH 后迟发性脑血맜痉挛的发生。
关键词: 迟发性脑血맜痉挛, 白细胞介素-8, 逆转录-聚合酶链反应, 蛛网膜下腔出血
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