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. 1983;2(4):599–603. doi: 10.1002/j.1460-2075.1983.tb01469.x

Distribution of ferritin receptors and coated pits on giant HeLa cells.

M S Bretscher, J N Thomson
PMCID: PMC555067  PMID: 6138252

Abstract

HeLa cells bind horse spleen ferritin when the two are incubated at 0 degrees C. Since the majority of this bound ferritin is located in coated pits, we conclude that the ferritin binds to a specific receptor which takes part in an endocytic cycle. When substrate-attached and well-spread giant HeLa cells are briefly labelled at 0 degrees C with ferritin, ferritin particles are found to be concentrated towards the cell periphery, where they exist largely outside coated pits. This peripheral concentration is a property of circulating (and not just newly synthesized) receptors because it is not affected by prior incubation of giant cells in cycloheximide. However, coated pits are themselves roughly uniformly distributed over the surface of these cells. These results provide evidence that the membrane internalised by coated pits on these cells is returned to the cell surface at the leading edge of the cell. Because of this separation of the sites of endocytosis and exocytosis, a flow of membrane must occur across the cell surface. This flow is composed of lipid plus receptors. The implications of this for capping and for cell spreading are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abercrombie M., Heaysman J. E., Pegrum S. M. The locomotion of fibroblasts in culture. 3. Movements of particles on the dorsal surface of the leading lamella. Exp Cell Res. 1970 Oct;62(2):389–398. doi: 10.1016/0014-4827(70)90570-7. [DOI] [PubMed] [Google Scholar]
  2. Anderson R. G., Brown M. S., Goldstein J. L. Inefficient internalization of receptor-bound low density lipoprotein in human carcinoma A-431 cells. J Cell Biol. 1981 Feb;88(2):441–452. doi: 10.1083/jcb.88.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson R. G., Brown M. S., Goldstein J. L. Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts. Cell. 1977 Mar;10(3):351–364. doi: 10.1016/0092-8674(77)90022-8. [DOI] [PubMed] [Google Scholar]
  4. Bleil J. D., Bretscher M. S. Transferrin receptor and its recycling in HeLa cells. EMBO J. 1982;1(3):351–355. doi: 10.1002/j.1460-2075.1982.tb01173.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bretscher M. S. Directed lipid flow in cell membranes. Nature. 1976 Mar 4;260(5546):21–23. doi: 10.1038/260021a0. [DOI] [PubMed] [Google Scholar]
  6. Bretscher M. S. Distribution of receptors for transferrin and low density lipoprotein on the surface of giant HeLa cells. Proc Natl Acad Sci U S A. 1983 Jan;80(2):454–458. doi: 10.1073/pnas.80.2.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bretscher M. S. Surface uptake by fibroblasts and its consequences. Cold Spring Harb Symp Quant Biol. 1982;46(Pt 2):707–712. doi: 10.1101/sqb.1982.046.01.067. [DOI] [PubMed] [Google Scholar]
  8. Bretscher M. S., Thomson J. N., Pearse B. M. Coated pits act as molecular filters. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4156–4159. doi: 10.1073/pnas.77.7.4156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. CIECIURA S. J., MARCUS P. I., PUCK T. T. Clonal growth in vitro of epithelial cells from normal human tissues. J Exp Med. 1956 Oct 1;104(4):615–628. doi: 10.1084/jem.104.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FAWCETT D. W. SURFACE SPECIALIZATIONS OF ABSORBING CELLS. J Histochem Cytochem. 1965 Feb;13:75–91. doi: 10.1177/13.2.75. [DOI] [PubMed] [Google Scholar]
  11. Goldstein J. L., Anderson R. G., Brown M. S. Coated pits, coated vesicles, and receptor-mediated endocytosis. Nature. 1979 Jun 21;279(5715):679–685. doi: 10.1038/279679a0. [DOI] [PubMed] [Google Scholar]
  12. Lagunoff D., Curran D. E. Role of bristle-coated membrane in the uptake of ferritin by rat macrophages. Exp Cell Res. 1972 Dec;75(2):337–346. doi: 10.1016/0014-4827(72)90438-7. [DOI] [PubMed] [Google Scholar]
  13. MARCUS P. I. Dynamics of surface modification in myxovirus-infected cells. Cold Spring Harb Symp Quant Biol. 1962;27:351–365. doi: 10.1101/sqb.1962.027.001.033. [DOI] [PubMed] [Google Scholar]
  14. Pearse B. M., Bretscher M. S. Membrane recycling by coated vesicles. Annu Rev Biochem. 1981;50:85–101. doi: 10.1146/annurev.bi.50.070181.000505. [DOI] [PubMed] [Google Scholar]
  15. TOLMACH L. J., MARCUS P. I. Development of x-ray induced giant HeLa cells. Exp Cell Res. 1960 Aug;20:350–360. doi: 10.1016/0014-4827(60)90163-4. [DOI] [PubMed] [Google Scholar]

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