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. 1983;2(2):277–282. doi: 10.1002/j.1460-2075.1983.tb01417.x

Observations concerning the discontinuous DNAs of cauliflower mosaic virus.

H Guilley 1, K E Richards 1, G Jonard 1
PMCID: PMC555125  PMID: 11894938

Abstract

The double-stranded circular DNA encapsidated within cauliflower mosaic virus (CaMV) particles contains three single-stranded discontinuities, two in one strand and one in the other, so that, upon denaturation, three linear single-stranded DNAs are produced. Here we show that a fourth much smaller single-stranded DNA, termed alpha1, is also present in denatured CaMV DNA preparations. The 5' extremity of alpha1 is identical to that of the alpha-strand, the strand of DNA possessing only one interruption, while its 3' extremity lies just two nucleotides downstream from a major transcription initiation site. We also show that the interrupted strand at each discontinuity sometimes has a single ribonucleotide in place of a deoxyribonucleotide at its 5' extremity. Oligoribonucleotide chains of eight and 10 residues in length have also been detected at the 5' end of one of the discontinuities. These structures are thought to be the vestiges of primers which have not been completely excised prior to encapsidation of the DNA. The possibility that synthesis of the alpha-strand occurs by reverse transcription of viral RNA using initiator tRNA(met) as primer is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson S., DePamphilis M. L. Metabolism of Okazaki fragments during simian virus 40 DNA replication. J Biol Chem. 1979 Nov 25;254(22):11495–11504. [PubMed] [Google Scholar]
  2. Covey S. N., Lomonossoff G. P., Hull R. Characterisation of cauliflower mosaic virus DNA sequences which encode major polyadenylated transcripts. Nucleic Acids Res. 1981 Dec 21;9(24):6735–6747. doi: 10.1093/nar/9.24.6735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Franck A., Guilley H., Jonard G., Richards K., Hirth L. Nucleotide sequence of cauliflower mosaic virus DNA. Cell. 1980 Aug;21(1):285–294. doi: 10.1016/0092-8674(80)90136-1. [DOI] [PubMed] [Google Scholar]
  4. Guilley H., Dudley R. K., Jonard G., Balàzs E., Richards K. E. Transcription of Cauliflower mosaic virus DNA: detection of promoter sequences, and characterization of transcripts. Cell. 1982 Oct;30(3):763–773. doi: 10.1016/0092-8674(82)90281-1. [DOI] [PubMed] [Google Scholar]
  5. Hay R. T., DePamphilis M. L. Initiation of SV40 DNA replication in vivo: location and structure of 5' ends of DNA synthesized in the ori region. Cell. 1982 Apr;28(4):767–779. doi: 10.1016/0092-8674(82)90056-3. [DOI] [PubMed] [Google Scholar]
  6. Hull R., Covey S. N., Stanley J., Davies J. W. The polarity of the cauliflower mosaic virus genome. Nucleic Acids Res. 1979 Oct 10;7(3):669–677. doi: 10.1093/nar/7.3.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hull R., Howell S. H. Structure of the cauliflower mosaic virus genome. II. Variation in DNA structure and sequence between isolates. Virology. 1978 May 15;86(2):482–493. doi: 10.1016/0042-6822(78)90087-9. [DOI] [PubMed] [Google Scholar]
  8. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RANDERATH K., RANDERATH E. ION-EXCHANGE CHROMATOGRAPHY OF NUCLEOTIDES ON POLY-(ETHYLENEIMINE)-CELLULOSE THIN LAYERS. J Chromatogr. 1964 Oct;16:111–125. doi: 10.1016/s0021-9673(01)82445-6. [DOI] [PubMed] [Google Scholar]
  10. Richards K. E., Guilley H., Jonard G. Further characterization of the discontinuities in cauliflower mosaic virus DNA. FEBS Lett. 1981 Nov 2;134(1):67–70. doi: 10.1016/0014-5793(81)80552-2. [DOI] [PubMed] [Google Scholar]
  11. Summers J., Mason W. S. Replication of the genome of a hepatitis B--like virus by reverse transcription of an RNA intermediate. Cell. 1982 Jun;29(2):403–415. doi: 10.1016/0092-8674(82)90157-x. [DOI] [PubMed] [Google Scholar]
  12. Tseng B. Y., Erickson J. M., Goulian M. Initiator RNA of nascent DNA from animal cells. J Mol Biol. 1979 Apr 25;129(4):531–545. doi: 10.1016/0022-2836(79)90467-4. [DOI] [PubMed] [Google Scholar]
  13. Volovitch M., Drugeon C., Yot P. Studies on the single-stranded discontinuities of the cauliflower mosaic virus genome. Nucleic Acids Res. 1978 Aug;5(8):2913–2925. doi: 10.1093/nar/5.8.2913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weaver R. F., Weissmann C. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. Nucleic Acids Res. 1979 Nov 10;7(5):1175–1193. doi: 10.1093/nar/7.5.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]

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