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. 1983;2(3):303–307. doi: 10.1002/j.1460-2075.1983.tb01423.x

DNA repair dependent NAD+ metabolism is impaired in cells from patients with Fanconi's anemia.

H Klocker 1, B Auer 1, M Hirsch-Kauffmann 1, H Altmann 1, H J Burtscher 1, M Schweiger 1
PMCID: PMC555133  PMID: 11894942

Abstract

In vitro cultivated fibroblasts derived either from patients with Fanconi's anemia (FA) or from healthy probands were analyzed for their DNA repair-dependent NAD+ metabolism. No difference in NAD+ pools was found. NAD+ consumption after cell damage by u.v. irradiation was, however, significantly reduced in FA cells. Several FA cell lines had a lowered ability to transfer ADP-ribose to acid-precipitable material. Additionally, a decreased activity of NAD: protein ADP-ribosyltransferase was found for three FA cell lines. Our data indicate, that FA is accompanied by a defective NAD+ metabolism during DNA repair.

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Selected References

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