Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1983;2(3):333–338. doi: 10.1002/j.1460-2075.1983.tb01427.x

The pattern of expression of chick delta-crystallin genes in lens differentiation and in trans-differentiating cultured tissues.

D J Bower, L H Errington, B J Pollock, S Morris, R M Clayton
PMCID: PMC555137  PMID: 11894946

Abstract

During development of the vertebrate lens, the lens epithelium undergoes a final stage of differentiation into lens fibre cells. Lens fibre cells can also be produced by trans-differentiation from certain extralenticular structures, all of which are of different developmental origin from lens, including embryonic neural retina and retinal pigmented epithelium. Delta-crystallin is the major lens protein in the chick and appears first in development; it is the major product in trans-differentiated retina of younger embryos. In both normal differentiation and trans-differentiation an increase of delta-crystallin coding RNA is detectable in the nucleus of cells prior to their terminal differentiation into lens fibres. The increase in transcription of delta-crystallin genes accompanying final differentiation of lens fibres, appears to take place slightly in advance of an increase in the capacity to process and transport this mRNA to the cytoplasm.

Full text

PDF
333

Images in this article

334Fig. 2.
on p.334
335Fig. 3.
on p.335
335Fig. 4.
on p.335
336Fig. 5.
on p.336

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barabanov V. M. Obnaruzhenie delta-kristallinov v adenogipofize kurinykh émbrionov. Dokl Akad Nauk SSSR. 1977 May-Jun;234(1):195–198. [PubMed] [Google Scholar]
  2. Bours J., Van Doorenmaalen W. J. Th presence of lens antigens in the intra-ocular tissues of the chick eye. Exp Eye Res. 1972 May;13(3):236–247. doi: 10.1016/0014-4835(72)90105-4. [DOI] [PubMed] [Google Scholar]
  3. Bower D. J., Errington L. H., Wainwright N. R., Sime C., Morris S., Clayton R. M. Cytoplasmic RNA sequences complementary to cloned chick delta-crystallin cDNA show size heterogeneity. Biochem J. 1982 Feb 1;201(2):339–344. doi: 10.1042/bj2010339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cervera M., Dreyfuss G., Penman S. Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected HeLa cells. Cell. 1981 Jan;23(1):113–120. doi: 10.1016/0092-8674(81)90276-2. [DOI] [PubMed] [Google Scholar]
  5. Clayton R. M., Campbell J. C., Truman D. E. A re-examination of the organ specificity of lens antigens. Exp Eye Res. 1968 Jan;7(1):11–29. doi: 10.1016/s0014-4835(68)80022-3. [DOI] [PubMed] [Google Scholar]
  6. Clayton R. M., Thomson I., de Pomerai D. I. Relationship between crystallin mRNA expression in retina cells and their capacity to re-differentiate into lens cells. Nature. 1979 Dec 6;282(5739):628–629. doi: 10.1038/282628a0. [DOI] [PubMed] [Google Scholar]
  7. De Pomerai D. I., Pritchard D. J., Clayton R. M. Biochemical and immunological studies of lentoid formation in cultures of embryonic chick neural retina and day-old chick lens epithelium. Dev Biol. 1977 Oct 15;60(2):416–427. doi: 10.1016/0012-1606(77)90139-7. [DOI] [PubMed] [Google Scholar]
  8. Eguchi G., Okada T. S. Differentiation of lens tissue from the progeny of chick retinal pigment cells cultured in vitro: a demonstration of a switch of cell types in clonal cell culture. Proc Natl Acad Sci U S A. 1973 May;70(5):1495–1499. doi: 10.1073/pnas.70.5.1495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jackson J. F., Clayton R. M., Williamson R., Thomson I., Truman D. E., de Pomerai D. I. Sequence complexity and tissue distribution of chick lens crystallin mRNAs. Dev Biol. 1978 Aug;65(2):383–395. doi: 10.1016/0012-1606(78)90034-9. [DOI] [PubMed] [Google Scholar]
  10. Jones R. E., Bhat S. P., Sullivan M. A., Piatigorsky J. Comparison of two delta-crystallin genes in the chicken. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5879–5883. doi: 10.1073/pnas.77.10.5879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MAISEL H., HARMISON C. AN IMMUNOEMBRYOLOGICAL STUDY OF THE CHICK IRIS. J Embryol Exp Morphol. 1963 Sep;11:483–491. [PubMed] [Google Scholar]
  12. Maitland N. J., Kinross J. H., Busuttil A., Ludgate S. M., Smart G. E., Jones K. W. The detection of DNA tumour virus-specific RNA sequences in abnormal human cervical biopsies by in situ hybridization. J Gen Virol. 1981 Jul;55(Pt 1):123–137. doi: 10.1099/0022-1317-55-1-123. [DOI] [PubMed] [Google Scholar]
  13. Okada T. S. Cellular metaplasia or transdifferentiation as a model for retinal cell differentiation. Curr Top Dev Biol. 1980;16:349–380. [PubMed] [Google Scholar]
  14. Okada T. S., Ito Y., Watanabe K., Eguchi G. Differentiation of lens in cultures of neural retinal cells of chick embryos. Dev Biol. 1975 Aug;45(2):318–329. doi: 10.1016/0012-1606(75)90069-x. [DOI] [PubMed] [Google Scholar]
  15. Papaconstantinou J. Molecular aspects of lens cell differentiation. Science. 1967 Apr 21;156(3773):338–346. doi: 10.1126/science.156.3773.338. [DOI] [PubMed] [Google Scholar]
  16. Piatigorsky J., Horwitz J., Simpson R. T. Partial dissociation and renaturation of embryonic chick delta-crystallin. Characterization by ultracentrifugation and circular dichroism. Biochim Biophys Acta. 1977 Feb 22;490(2):279–289. doi: 10.1016/0005-2795(77)90003-4. [DOI] [PubMed] [Google Scholar]
  17. Thomson I., de Pomerai D. I., Jackson J. F., Clayton R. M. Lens-specific mRNA in cultures of embryonic chick neural retina and pigmented epithelium. Exp Cell Res. 1979 Aug;122(1):73–81. doi: 10.1016/0014-4827(79)90562-7. [DOI] [PubMed] [Google Scholar]
  18. Williams L. A., Piatigorsky J. Comparative and evolutionary aspects of delta-crystallin in the vertebrate lens. Eur J Biochem. 1979 Oct 15;100(2):349–357. doi: 10.1111/j.1432-1033.1979.tb04177.x. [DOI] [PubMed] [Google Scholar]
  19. Zwaan J., Ikeda A. Macromolecular events during differentiation of the chicken lens. Exp Eye Res. 1968 Apr;7(2):301–311. doi: 10.1016/s0014-4835(68)80081-8. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES