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. Author manuscript; available in PMC: 2017 Aug 10.
Published in final edited form as: Virology. 2016 Apr 20;494:89–99. doi: 10.1016/j.virol.2016.04.013

Figure 5. A Valine at Position 358 in the PB2 gene of H7N3 attenuates the virus.

Figure 5

H7N3 viruses were generated containing single point mutations at position 62 (G62R), 358 (E358V), 471 (A471T) and 681 (E681G) of the H7N3 PB2 polymerase gene. Female DBA/2J mice were infected with 102 TCID50 of H7N3 viruses containing each of the four point mutations and mortality (A) and morbidity (B) was compared to H7N3 and H7N3 PB2N9 virus. Each virus strain was tested in two or more separate experiments and includes at least eight animals per strain of IAV. * = P<0.05; ** = P<0.01; *** = P<0.001.