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. 1983;2(6):947–952. doi: 10.1002/j.1460-2075.1983.tb01526.x

Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene

JAllan Downie 1,*, Qing-Sheng Ma 1, Celia D Knight 1, Gerd Hombrecher 1, Andrew WB Johnston 1
PMCID: PMC555213  PMID: 16453461

Abstract

The region of the Rhizobium leguminosarum plasmid pRL1JI involved in nodulation and nitrogen fixation has been cloned on a series of four overlapping cosmid clones. These clones represent ˜60 kb of pRL1JI DNA on which a series of Tn5-induced fix and nod alleles have been identified, with the two most distant alleles being separated by ˜45 kb of DNA. The mutant alleles fell into three groups, two clusters of fix alleles separated by one cluster of nod alleles. Within one group of fix alleles, DNA homologous to the nifA gene of Klebsiella pneumoniae has been identified, whereas the pRL1JI DNA homologous to the K. pneumoniae nitrogenase genes is present within the other group of fix alleles.

Keywords: Rhizobium, nitrogen fixation, nodulation, nifA gene

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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