Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1983;2(7):1123–1127. doi: 10.1002/j.1460-2075.1983.tb01556.x

tRNA-like structures of plant viral RNAs: conformational requirements for adenylation and aminoacylation.

R L Joshi, S Joshi, F Chapeville, A L Haenni
PMCID: PMC555245  PMID: 6556143

Abstract

Bromo- and cucumovirus RNAs contain a tRNA-like structure as an integral part of their genome. This structure is located at the 3' end of the viral RNA and is an acceptor of tyrosine. The 3' regions of representative viral RNAs have been sequenced and quite unorthodox secondary foldings have been proposed for these 3' ends. The question therefore remained as to how these structures could be recognized by tRNA-specific enzymes. We have established the minimum number of nucleotides from the 3' end of the brome mosaic virus and broad bean mottle virus RNAs required for the formation of structures recognized by the tyrosyl-tRNA synthetase and/or the tRNA nucleotidyltransferase. The results obtained delineate the length of the tRNA-like region, and indicate that the 5' region of the tRNA-like structure participates in the formation of the amino acid stem. This has led us to propose an 'L'-shaped secondary structure for these tRNA-like regions.

Full text

PDF
1123

Images in this article

1124Fig. 2.
on p.1124

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahlquist P., Dasgupta R., Kaesberg P. Near identity of 3- RNA secondary structure in bromoviruses and cucumber mosaic virus. Cell. 1981 Jan;23(1):183–189. doi: 10.1016/0092-8674(81)90283-x. [DOI] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Carre D. S., Litvak S., Chapeville F. Purification and properties of Escherichia coli CTP (ATP)-tRNA nucleotidyltransferase. Biochim Biophys Acta. 1970 Dec 14;224(2):371–381. doi: 10.1016/0005-2787(70)90570-8. [DOI] [PubMed] [Google Scholar]
  4. Dasgupta R., Kaesberg P. Sequence of an oligonucleotide derived from the 3' end of each of the four brome mosaic viral RNAs. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4900–4904. doi: 10.1073/pnas.74.11.4900. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deák F., Dénes G. Purification and some properties of rat liver tyrosyl-tRNA synthetase. Biochim Biophys Acta. 1978 Oct 12;526(2):626–634. doi: 10.1016/0005-2744(78)90153-5. [DOI] [PubMed] [Google Scholar]
  6. Florentz C., Briand J. P., Romby P., Hirth L., Ebel J. P., Glegé R. The tRNA-like structure of turnip yellow mosaic virus RNA: structural organization of the last 159 nucleotides from the 3' OH terminus. EMBO J. 1982;1(2):269–276. doi: 10.1002/j.1460-2075.1982.tb01158.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gauss D. H., Sprinzl M. Compilation of tRNA sequences. Nucleic Acids Res. 1983 Jan 11;11(1):r1–53. [PMC free article] [PubMed] [Google Scholar]
  8. Giegé R., Briand J. P., Mengual R., Ebel J. P., Hirth L. Valylation of the two RNA components of turnip-yellow mosaic virus and specificity of the tRNA aminoacylation reaction. Eur J Biochem. 1978 Mar;84(1):251–256. doi: 10.1111/j.1432-1033.1978.tb12163.x. [DOI] [PubMed] [Google Scholar]
  9. Haenni A. L., Joshi S., Chapeville F. tRNA-like structures in the genomes of RNA viruses. Prog Nucleic Acid Res Mol Biol. 1982;27:85–104. doi: 10.1016/s0079-6603(08)60598-x. [DOI] [PubMed] [Google Scholar]
  10. Hall T. C., Shih D. S., Kaesberg P. Enzyme-mediated binding of tyrosine to brome-mosaic-virus ribonucleic acid. Biochem J. 1972 Oct;129(4):969–976. doi: 10.1042/bj1290969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Joshi S., Chapeville F., Haenni A. L. Length requirements for tRNA-specific enzymes and cleavage specificity at the 3' end of turnip yellow mosaic virus RNA. Nucleic Acids Res. 1982 Mar 25;10(6):1947–1962. doi: 10.1093/nar/10.6.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Joshi S., Chapeville F., Haenni A. L. Turnip yellow mosaic virus RNA is aminoacylated in vivo in Chinese cabbage leaves. EMBO J. 1982;1(8):935–938. doi: 10.1002/j.1460-2075.1982.tb01274.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Joshi S., Haenni A. L. Fluorographic detection of nucleic acids labelled with weak beta-emitters in gels containing high acrylamide concentrations. FEBS Lett. 1980 Aug 25;118(1):43–46. doi: 10.1016/0014-5793(80)81214-2. [DOI] [PubMed] [Google Scholar]
  14. Joshi S., Haenni A. L., Hubert E., Huez G., Marbaix G. In vivo aminoacylation and 'processing' of turnip yellow mosaic virus RNA in Xenopus laevis oocytes. Nature. 1978 Sep 28;275(5678):339–341. doi: 10.1038/275339a0. [DOI] [PubMed] [Google Scholar]
  15. Kim S. H. Three-dimensional structure of transfer RNA. Prog Nucleic Acid Res Mol Biol. 1976;17:181–216. doi: 10.1016/s0079-6603(08)60070-7. [DOI] [PubMed] [Google Scholar]
  16. Kohl R. J., Hall T. C. Aminoacylation of RNA from several viruses: amino acid specificity and differential activity of plant, yeast and bacterial synthetases. J Gen Virol. 1974 Nov;25(2):257–261. doi: 10.1099/0022-1317-25-2-257. [DOI] [PubMed] [Google Scholar]
  17. Rietveld K., Van Poelgeest R., Pleij C. W., Van Boom J. H., Bosch L. The tRNA-like structure at the 3' terminus of turnip yellow mosaic virus RNA. Differences and similarities with canonical tRNA. Nucleic Acids Res. 1982 Mar 25;10(6):1929–1946. doi: 10.1093/nar/10.6.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schimmel P. R., Söll D. Aminoacyl-tRNA synthetases: general features and recognition of transfer RNAs. Annu Rev Biochem. 1979;48:601–648. doi: 10.1146/annurev.bi.48.070179.003125. [DOI] [PubMed] [Google Scholar]
  19. Shih D. S., Dasgupta R., Kaesberg P. 7-Methyl-guanosine and efficiency of RNA translation. J Virol. 1976 Aug;19(2):637–642. doi: 10.1128/jvi.19.2.637-642.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Solari A., Gatica M., Allende J. E. In vivo repair of the 3'terminus of transfer RNA injected into amphibian oocytes. Nucleic Acids Res. 1977 Jun;4(6):1873–1880. doi: 10.1093/nar/4.6.1873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tinoco I., Jr, Uhlenbeck O. C., Levine M. D. Estimation of secondary structure in ribonucleic acids. Nature. 1971 Apr 9;230(5293):362–367. doi: 10.1038/230362a0. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES