Table 1.
Strains and plasmids
| Strains or plasmids | Description | Source |
|---|---|---|
| Strains | ||
| Aeromonas hydrophilaB11 | Wild‐type strain (SmR) | (Qin et al., 2014) |
| AM01~AM102 | Mini‐Tn10Km insertion mutant (SmRKmR) | Our study |
| AM73 (acuC−) | AcuC:: mini‐Tn10Km (SmRKmR) | Our study |
| AC73 (acuC+) | AM73 complemented with pACYC184‐AcuC (SmRKmRCmr) | Our study |
| E. coliSM10 | thithrleutonAlacYsupErecARP4 – 2 ‐ Tc:: Mu:: Km (λpir) | (Qin et al., 2013) |
| E. coliDH5α | F−, φ 80dlacZΔM15, ΔU169 (lacZYA‐argF), deoR, recA1endA1, hsdR17 (rK − ,mK + ), phoA, supE44, λ− , thi ‐1, gyrA96, relA1 | Takara (Qin et al., 2014) |
| Plasmids | ||
| pMD18 ‐ T | Cloning vector (ApR) | Takara |
| pLOF/Km | Tnl0‐connected transmit plasmid (KmRApR); | (Herrero et al., 1990) |
| pACYC184 | (CmRTcR) | afforded by Prof. Nie |
| pACYC184 ‐acuC | Recombination of pACYC184 and AcuC including the promoter and ORF (CmR) | Our study |