Table 2.
Quantification of bacterial counts in water samples
| A | Total viable count [cfu.cm−3] | |||
|---|---|---|---|---|
| PM1 | PM2 | PM3 | PM4 | |
| Clear filtrate | >103 | >103 | >103 | >104 |
| White water | >104 | >105 | >104 | >104 |
| Press water | >104 | >104 | >105 | >104 |
| B | Total DNA [genome equivalents cm−3] | |||
|---|---|---|---|---|
| PM1 | PM2 | PM3 | PM4 | |
| Clear filtrate | 2·103 | 4·103 | 6·104 | 5·103 |
| White water | 3·104 | 3·104 | 1·105 | 4·104 |
| Press water | 3·103 | 1·104 | 5·103 | 5·103 |
| C | PMA‐treated DNA (live) [genome equivalents cm−3] | |||
|---|---|---|---|---|
| PM1 | PM2 | PM3 | PM4 | |
| Clear filtrate | 1·103 | 3·103 | 6·103 | 5·103 |
| White water | 1·104 | 2·104 | 1·104 | 3·103 |
| Press water | 8·102 | 1·104 | 3·103 | 2·103 |
(A) Total viable count as colony‐forming units (cfu) per cm3. (B) Total bacterial DNA. (C) DNA from live bacteria. For live fraction, the samples were PMA‐treated prior DNA isolation and quantification to remove DNA from dead bacteria. Bacterial DNA was quantified by 16S real‐time PCR relative to a standard consisting of genomic DNA equivalents of E. coli K1.