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. 1983;2(9):1585–1589. doi: 10.1002/j.1460-2075.1983.tb01628.x

Differential regulation of HLA-DR mRNAs and cell surface antigens by interferon.

F Rosa 1, D Hatat 1, A Abadie 1, D Wallach 1, M Revel 1, M Fellous 1
PMCID: PMC555327  PMID: 11892816

Abstract

Human interferons-alpha, -beta and -gamma enhance HLA-DR mRNAs in all the human lymphoblastoid and melanoma cell lines studied. The increase concerns both alpha and beta chain mRNAs. Moreover, we show that immune interferon-gamma preferentially enhances class II MHC mRNA. This effect of IFN-gamma on the synthesis of alpha and beta HLA-DR chains has been also analysed by immunoprecipitation. It is abolished by a monoclonal antibody directed against human IFN-gamma. The effect of interferon on the cell surface level of HLA-DR molecules does not always correspond to the enhancement of HLA-DR mRNA. Our experiments suggest that this discrepancy between the enhancement of HLA-DR mRNA and cell surface antigen might be due to a constitutively high level of the corresponding antigens on several of the human cells studied.

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