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SDS-PAGE showing the expression of the ChiA1-2 gene using BL21ChiA1-2Ll1 (A) and BL21ChiA1-2Ll2 (B) strains induced with IPTG. Lane 1: molecular weight protein marker (A: Hide Range Sigma Marker™, Sigma-Aldrich; B: Protein Marker, Broad Range, Biolabs); lane 2: cell extract before induction; lanes 3–4: cell extract 2 and 4 h, respectively, after induction. The recombinant protein fused with the GST tag is boxed.
