Table 1.

Effect of the promoters and different induction conditions on the expression of the active form of the chitinase encoded by the ChiA1-2 gene.

Activity (U/mL)
Parameter analyzed	BL21ChiA1-2Ll1a	BL21ChiA1-2Ll2b
OD600 nm
0.5	240.7 ± 0.4	38.4 ± 2.7
0.8	98.7 ±2 .4	55.8 ± 1.1
1.2	95.1 ± 0.8	66.3 ± 4
IPTG concentration (mM)
0.12	89.4 ± 5	62 ± 4.3
0.25	108 ± 0.4	56.8 ± 1.2
0.5	98.7 ± 1.5	55.8 ± 1.1
Post-induction time (h)
1	152.7 ± 6	45.2 ± 0.9
5	98.6 ± 0.5	55.8 ± 2.8
24	66.2 ± 5.2	33 ± 0.4

^aExpressing the gene from the tac promoter.

^bExpressing the gene from the T7 promoter.