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. 1983;2(10):1715–1719. doi: 10.1002/j.1460-2075.1983.tb01647.x

Avidin is induced in chicken embryo fibroblasts by viral transformation and cell damage.

J Korpela, M Kulomaa, P Tuohimaa, A Vaheri
PMCID: PMC555348  PMID: 6315397

Abstract

Synthesis and secretion of avidin was studied in cultured chicken embryo fibroblasts infected with transforming retroviruses (Rous sarcoma virus, its mutants temperature-sensitive for transformation, OK-10 virus) or a nontransforming retrovirus (RAV-1). Avidin was detectable in both transformed and untransformed cultures, and was identical to chicken egg white avidin by several criteria: biotin-binding, heat-induced biotin exchange, subunit size (mol. wt. 15 600), immunoprecipitation of metabolically labeled proteins and immunoblotting. Transformation increased the production of avidin up to 50-fold, but several experiments suggested that the induction was not a direct consequence of virus-induced cell transformation. The production of avidin seemed to relate to cellular damage both in cultures of virus-transformed and of normal fibroblasts. It may represent a response to cellular damage and viral transformation may activate the process.

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Selected References

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