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. 1983;2(10):1753–1757. doi: 10.1002/j.1460-2075.1983.tb01653.x

A transformation system for the hypotrichous ciliate Stylonychia mytilus

Ingrid U Wünning 1,*, Hans J Lipps 1
PMCID: PMC555354  PMID: 16453475

Abstract

We describe a transformation system for the ciliate Stylonychia mytilus. The neomycin resistance gene from Escherichia coli transposon Tn5, which codes for the enzyme phosphotransferase and confers resistance to the antibiotic G 418, was ligated into macronuclear `gene-size' DNA molecules. Using this recombinant DNA for transformation experiments we show that the gene is replicated and expressed in transformed cells.

Keywords: aminoglycoside G 418 resistance, gene-sized DNA

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Selected References

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