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. 1983;2(10):1791–1794. doi: 10.1002/j.1460-2075.1983.tb01659.x

Easy identification of cDNA clones.

U Rüther, B Müller-Hill
PMCID: PMC555360  PMID: 6315402

Abstract

A set of six cloning vectors, pUR 278, 288, 289, 290, 291, 292 is presented. These vectors have the cloning sites, BamHI, SalI, PstI, XbaI and HindIII, in all frames at the 3' end of the lacZ gene. Insertion of cDNA in the proper cloning sites leads to a fusion protein of active beta-galactosidase and the peptide encoded by the cDNA. A simple immunoenzymatic assay can be used to identify clones in such a cDNA library.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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