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. 1983;2(10):1845–1852. doi: 10.1002/j.1460-2075.1983.tb01668.x

Structural features of B family chorion sequences in the silkmoth Bombyx mori, and their evolutionary implications.

S G Tsitilou, G C Rodakis, M Alexopoulou, F C Kafatos, K Ito, K Iatrou
PMCID: PMC555369  PMID: 6571700

Abstract

Partial protein sequences, and DNA sequences of corresponding cDNA and genomic clones were obtained and analyzed to reveal the primary structural features of major, developmentally middle or late components of the B chorion multigene family in Bombyx mori. Comparisons with other types of sequences confirm and clarify the tripartite domain structure of chorion proteins. Glycine-, leucine- and tyrosine-containing, tandemly repetitive peptides form the bulk of the amino-terminal and carboxy-terminal domains ('arms'). Extensive sequence homologies suggest a common evolutionary origin for the amino-terminal arms of some B. mori B sequences and the corresponding portions of members of a different (A) chorion multigene family in Antheraea polyphemus, a distantly related silkmoth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eickbush T. H., Kafatos F. C. A walk in the chorion locus of Bombyx mori. Cell. 1982 Jun;29(2):633–643. doi: 10.1016/0092-8674(82)90179-9. [DOI] [PubMed] [Google Scholar]
  2. Hamodrakas S. J., Asher S. A., Mazur G. D., Regier J. C., Kafatos F. C. Laser Raman studies of protein conformation in the silkmoth chorion. Biochim Biophys Acta. 1982 May 3;703(2):216–222. doi: 10.1016/0167-4838(82)90051-6. [DOI] [PubMed] [Google Scholar]
  3. Iatrou K., Tsitilou S. G. Coordinately expressed chorion genes of Bombyx mori: is developmental specificity determined by secondary structure recognition? EMBO J. 1983;2(9):1431–1440. doi: 10.1002/j.1460-2075.1983.tb01604.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Iatrou K., Tsitilou S. G., Goldsmith M. R., Kafatos F. C. Molecular analysis of the GrB mutation in Bombyx mori through the use of chorion cDNA library. Cell. 1980 Jul;20(3):659–669. doi: 10.1016/0092-8674(80)90312-8. [DOI] [PubMed] [Google Scholar]
  5. Iatrou K., Tsitilou S. G., Kafatos F. C. Developmental classes and homologous families of chorion genes in Bombyx mori. J Mol Biol. 1982 May 25;157(3):417–434. doi: 10.1016/0022-2836(82)90469-7. [DOI] [PubMed] [Google Scholar]
  6. Jones C. W., Kafatos F. C. Accepted mutations in a gene family: evolutionary diversification of duplicated DNA. J Mol Evol. 1982;19(1):87–103. doi: 10.1007/BF02100227. [DOI] [PubMed] [Google Scholar]
  7. Jones C. W., Kafatos F. C. Coordinately expressed members of two chorion multi-gene families are clustered, alternating and divergently orientated. Nature. 1980 Apr 17;284(5757):635–638. doi: 10.1038/284635a0. [DOI] [PubMed] [Google Scholar]
  8. Jones C. W., Rosenthal N., Rodakis G. C., Kafatos F. C. Evolution of two major chorion multigene families as inferred from cloned cDNA and protein sequences. Cell. 1979 Dec;18(4):1317–1332. doi: 10.1016/0092-8674(79)90242-3. [DOI] [PubMed] [Google Scholar]
  9. Jones C. W., Rosenthal N., Rodakis G. C., Kafatos F. C. Evolution of two major chorion multigene families as inferred from cloned cDNA and protein sequences. Cell. 1979 Dec;18(4):1317–1332. doi: 10.1016/0092-8674(79)90242-3. [DOI] [PubMed] [Google Scholar]
  10. Kafatos F. C., Regier J. C., Mazur G. D., Nadel M. R., Blau H. M., Petri W. H., Wyman A. R., Gelinas R. E., Moore P. B., Paul M. The eggshell of insects: differentiation-specific proteins and the control of their synthesis and accumulation during development. Results Probl Cell Differ. 1977;8:45–145. doi: 10.1007/978-3-540-37332-2_2. [DOI] [PubMed] [Google Scholar]
  11. Lecanidou R., Eickbush T. H., Rodakis G. C., Kafatos F. C. Novel B family sequence from an early chorion cDNA library of Bombyx mori. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1955–1959. doi: 10.1073/pnas.80.7.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nadel M. R., Thireos G., Kafatos F. C. Effect of the pleiotropic GrB mutation of Bombyx mori on chorion protein synthesis. Cell. 1980 Jul;20(3):649–658. doi: 10.1016/0092-8674(80)90311-6. [DOI] [PubMed] [Google Scholar]
  14. Paul M., Kafatos F. C., Regier J. C. A comparative study of eggshell proteins in lepidoptera. J Supramol Struct. 1972;1(1):60–65. doi: 10.1002/jss.400010109. [DOI] [PubMed] [Google Scholar]
  15. Regier J. C., Kafatos F. C., Goodfliesh R., Hood L. Silkmoth chorion proteins: sequence analysis of the products of a multigene family. Proc Natl Acad Sci U S A. 1978 Jan;75(1):390–394. doi: 10.1073/pnas.75.1.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Regier J. C., Kafatos F. C., Hamodrakas S. J. Silkmoth chorion multigene families constitute a superfamily: comparison of C and B family sequences. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1043–1047. doi: 10.1073/pnas.80.4.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Regier J. C., Kafatos F. C. In vivo kinetics of pyrrolidonecarboxylic acid formation in selected silkmoth chorion proteins. J Biol Chem. 1981 Jun 25;256(12):6444–6451. [PubMed] [Google Scholar]
  18. Regier J. C., Kafatos F. C., Kramer K. J., Heinrikson R. L., Keim P. S. Silkmoth chorion proteins. Their diversity, amino acid composition, and the NH-terminal sequence of one component. J Biol Chem. 1978 Feb 25;253(4):1305–1314. [PubMed] [Google Scholar]
  19. Regier J. C., Mazur G. D., Kafatos F. C., Paul M. Morphogenesis of silkmoth chorion: initial framework formation and its relation to synthesis of specific proteins. Dev Biol. 1982 Jul;92(1):159–174. doi: 10.1016/0012-1606(82)90160-9. [DOI] [PubMed] [Google Scholar]
  20. Rodakis G. C., Kafatos F. C. Origin of evolutionary novelty in proteins: how a high-cysteine chorion protein has evolved. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3551–3555. doi: 10.1073/pnas.79.11.3551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rodakis G. C., Moschonas N. K., Kafatos F. C. Evolution of a multigene family of chorion proteins in silkmoths. Mol Cell Biol. 1982 May;2(5):554–563. doi: 10.1128/mcb.2.5.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tsitilou S. G., Regier J. C., Kafatos F. C. Selection and sequence analysis of a cDNA clone encoding a known chorion protein of the A family. Nucleic Acids Res. 1980 May 10;8(9):1987–1997. doi: 10.1093/nar/8.9.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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