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. 1983;2(11):1937–1941. doi: 10.1002/j.1460-2075.1983.tb01682.x

Appearance of functional EGF receptor kinase during rodent embryogenesis.

M Hortsch, J Schlessinger, E Gootwine, C G Webb
PMCID: PMC555391  PMID: 6196193

Abstract

Mouse and rat embryonic tissues at various stages of development were examined for epidermal growth factor (EGF) receptor kinase activity. The phosphorylated EGF receptor from embryonic tissues appeared as a band of mol. wt. 170 000 daltons on SDS gels. It was clearly demonstrable in the developing mouse fetus from 10 days of gestation onwards. The distribution of the EGF receptor kinase was studied in various tissues of 13 day mouse fetuses. The activity was apparent in the skin, developing skeletal muscles and various internal organs but was notably absent in the liver and brain. The amnion was found to be one of the richest sources of activity while the yolk sac was negative, and the placenta was weakly positive. In 16 day rat fetuses the distribution was quite similar to that of the 13 day mouse fetus. The liver acquired EGF receptor kinase activity by 18 days of gestation and had high activity in neonates. Phosphoamino acid analysis revealed that phosphotyrosine was the major labelled amino acid residue in the embryonic tissues. Thus, the EGF receptor of fetal tissues as studied by immune precipitation and phosphorylation appears to be a similar entity to that found in adult mammalian tissues. This functional EGF receptor kinase activity could first be detected at the time of onset of organogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamson E. D., Deller M. J., Warshaw J. B. Functional EGF receptors are present on mouse embryo tissues. Nature. 1981 Jun 25;291(5817):656–659. doi: 10.1038/291656a0. [DOI] [PubMed] [Google Scholar]
  2. Adamson E. D., Warshaw J. B. Down-regulation of epidermal growth factor receptors in mouse embryos. Dev Biol. 1982 Apr;90(2):430–434. doi: 10.1016/0012-1606(82)90392-x. [DOI] [PubMed] [Google Scholar]
  3. BALLARD F. J., OLIVER I. T. Glycogen metabolism in embryonic chick and neonatal rat liver. Biochim Biophys Acta. 1963 Jun 4;71:578–588. doi: 10.1016/0006-3002(63)91130-2. [DOI] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. COHEN S. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962 May;237:1555–1562. [PubMed] [Google Scholar]
  6. Carpenter G., Cohen S. Epidermal growth factor. Annu Rev Biochem. 1979;48:193–216. doi: 10.1146/annurev.bi.48.070179.001205. [DOI] [PubMed] [Google Scholar]
  7. Carpenter G., King L., Jr, Cohen S. Epidermal growth factor stimulates phosphorylation in membrane preparations in vitro. Nature. 1978 Nov 23;276(5686):409–410. doi: 10.1038/276409a0. [DOI] [PubMed] [Google Scholar]
  8. Cassel D., Glaser L. Proteolytic cleavage of epidermal growth factor receptor. A Ca2+-dependent, sulfhydryl-sensitive proteolytic system in A431 cells. J Biol Chem. 1982 Aug 25;257(16):9845–9848. [PubMed] [Google Scholar]
  9. Catterton W. Z., Escobedo M. B., Sexson W. R., Gray M. E., Sundell H. W., Stahlman M. T. Effect of epidermal growth factor on lung maturation in fetal rabbits. Pediatr Res. 1979 Feb;13(2):104–108. doi: 10.1203/00006450-197902000-00004. [DOI] [PubMed] [Google Scholar]
  10. Cohen S., Carpenter G., King L., Jr Epidermal growth factor-receptor-protein kinase interactions. Co-purification of receptor and epidermal growth factor-enhanced phosphorylation activity. J Biol Chem. 1980 May 25;255(10):4834–4842. [PubMed] [Google Scholar]
  11. Cohen S., Fava R. A., Sawyer S. T. Purification and characterization of epidermal growth factor receptor/protein kinase from normal mouse liver. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6237–6241. doi: 10.1073/pnas.79.20.6237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Cohen S. The stimulation of epidermal proliferation by a specific protein (EGF). Dev Biol. 1965 Dec;12(3):394–407. doi: 10.1016/0012-1606(65)90005-9. [DOI] [PubMed] [Google Scholar]
  13. Gospodarowicz D. Epidermal and nerve growth factors in mammalian development. Annu Rev Physiol. 1981;43:251–263. doi: 10.1146/annurev.ph.43.030181.001343. [DOI] [PubMed] [Google Scholar]
  14. Gospodarowicz D., Moran J. S. Growth factors in mammalian cell culture. Annu Rev Biochem. 1976;45:531–558. doi: 10.1146/annurev.bi.45.070176.002531. [DOI] [PubMed] [Google Scholar]
  15. Haigler H. T., Carpenter G. Production and characterization of antibody blocking epidermal growth factor:receptor interactions. Biochim Biophys Acta. 1980 May 23;598(2):314–325. doi: 10.1016/0005-2736(80)90009-7. [DOI] [PubMed] [Google Scholar]
  16. Hassell J. R. The development of rat palatal shelves in vitro. An ultrastructural analysis of the inhibition of epithelial cell death and palate fusion by the epidermal growth factor. Dev Biol. 1975 Jul;45(1):90–102. doi: 10.1016/0012-1606(75)90244-4. [DOI] [PubMed] [Google Scholar]
  17. Hock R. A., Nexø E., Hollenberg M. D. Isolation of the human placenta receptor for epidermal growth factor-urogastrone. Nature. 1979 Feb 1;277(5695):403–405. doi: 10.1038/277403a0. [DOI] [PubMed] [Google Scholar]
  18. Hunter T., Sefton B. M. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311–1315. doi: 10.1073/pnas.77.3.1311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  20. Nexø E., Hollenberg M. D., Figueroa A., Pratt R. M. Detection of epidermal growth factor-urogastrone and its receptor during fetal mouse development. Proc Natl Acad Sci U S A. 1980 May;77(5):2782–2785. doi: 10.1073/pnas.77.5.2782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. O'Keefe E., Hollenberg M. D., Cuatrecasas P. Epidermal growth factor. Characteristics of specific binding in membranes from liver, placenta, and other target tissues. Arch Biochem Biophys. 1974 Oct;164(2):518–526. doi: 10.1016/0003-9861(74)90062-9. [DOI] [PubMed] [Google Scholar]
  22. Rees A. R., Adamson E. D., Graham C. F. Epidermal growth factor receptors increase during the differentiation of embryonal carcinoma cells. Nature. 1979 Sep 27;281(5729):309–311. doi: 10.1038/281309a0. [DOI] [PubMed] [Google Scholar]
  23. Savage C. R., Jr, Inagami T., Cohen S. The primary structure of epidermal growth factor. J Biol Chem. 1972 Dec 10;247(23):7612–7621. [PubMed] [Google Scholar]
  24. Schlessinger J., Schreiber A. B., Levi A., Lax I., Libermann T., Yarden Y. Regulation of cell proliferation by epidermal growth factor. CRC Crit Rev Biochem. 1983;14(2):93–111. doi: 10.3109/10409238309102791. [DOI] [PubMed] [Google Scholar]
  25. Schreiber A. B., Lax I., Yarden Y., Eshhar Z., Schlessinger J. Monoclonal antibodies against receptor for epidermal growth factor induce early and delayed effects of epidermal growth factor. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7535–7539. doi: 10.1073/pnas.78.12.7535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Schreiber A. B., Libermann T. A., Lax I., Yarden Y., Schlessinger J. Biological role of epidermal growth factor-receptor clustering. Investigation with monoclonal anti-receptor antibodies. J Biol Chem. 1983 Jan 25;258(2):846–853. [PubMed] [Google Scholar]
  27. Twardzik D. R., Ranchalis J. E., Todaro G. J. Mouse embryonic transforming growth factors related to those isolated from tumor cells. Cancer Res. 1982 Feb;42(2):590–593. [PubMed] [Google Scholar]

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