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. 1983;2(11):1993–1999. doi: 10.1002/j.1460-2075.1983.tb01690.x

Purification and partial amino acid sequence analysis of the cellular tumour antigen, p53, from mouse SV40-transformed cells.

K Leppard, N Totty, M Waterfield, E Harlow, J Jenkins, L Crawford
PMCID: PMC555399  PMID: 6315411

Abstract

The cellular tumour antigen p53 is implicated in the transformation process. To compare p53 from transformed cells and their normal counterparts in detail, and so to identify any structural differences that might alter p53 function, requires information on the primary structure of the protein. By making use of immunochemical techniques we have been able to purify nanomole amounts of p53. This was sufficient, using high sensitivity automated gas-phase sequencing techniques to determine the amino acid sequence of two tryptic peptides from p53. Their sequences agree completely with the predicted polypeptide sequence derived from a cloned cDNA for p53 mRNA and provide the first data on the amino acid sequence of p53. A combination of the high sensitivity amino acid sequencing procedures used here and cDNA sequence analysis should provide the complete amino acid sequence of p53.

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1993

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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