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. 1983;2(12):2259–2263. doi: 10.1002/j.1460-2075.1983.tb01732.x

Isolation of cDNA clones for the human transferrin receptor.

C Schneider, M Kurkinen, M Greaves
PMCID: PMC555443  PMID: 6321157

Abstract

A cDNA clone bank containing 30 000 clones was constructed from sucrose gradient-fractionated mRNA from human placenta. mRNA coding for transferrin receptor (TR) was enriched by polysome immuno-adsorbed chromatography with monospecific rabbit IgG and protein-A Sepharose. The library was screened for hybridisation to 32P-labelled cDNA synthesised from immunoselected TR mRNA and from poly(A)+ RNA of the polysome fraction that failed to bind to protein-A Sepharose. Plasmids isolated from colonies showing hybridisation only to the probe made from immunoselected mRNA were then subjected to hybrid selection. Two clones, pTR-48 and pTR-67, were able to hybridise the mRNA coding for the TR.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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