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. 1983;2(12):2281–2283. doi: 10.1002/j.1460-2075.1983.tb01735.x

Localisation of the human N-ras oncogene to chromosome 1cen - p21 by in situ hybridisation.

M Davis, S Malcolm, A Hall, C J Marshall
PMCID: PMC555446  PMID: 6667677

Abstract

The N-ras gene is a transforming gene isolated from a variety of human tumour cell lines and is a member of a family of related ras genes. Somatic cell hybrids have previously shown that the N-ras gene is located on chromosome 1. We have confirmed this localisation by in situ hybridisation to metaphase preparations of lymphocytes and localised the gene to the region 1cen - p21. A survey has found 47 reported cases of malignancy involving deletions in the short arm of chromosome 1. Fifteen of the 47 involved a deletion in this region.

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Selected References

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  1. Barton P., Malcolm S., Murphy C., Ferguson-Smith M. A. Localization of the human alpha-globin gene cluster to the short arm of chromosome 16 (16p12-16pter) by hybridization in situ. J Mol Biol. 1982 Apr 5;156(2):269–278. doi: 10.1016/0022-2836(82)90328-x. [DOI] [PubMed] [Google Scholar]
  2. Brito-Babapulle V., Atkin N. B. Break points in chromosome #1 abnormalities of 218 human neoplasms. Cancer Genet Cytogenet. 1981 Nov;4(3):215–225. doi: 10.1016/0165-4608(81)90015-7. [DOI] [PubMed] [Google Scholar]
  3. Capon D. J., Chen E. Y., Levinson A. D., Seeburg P. H., Goeddel D. V. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue. Nature. 1983 Mar 3;302(5903):33–37. doi: 10.1038/302033a0. [DOI] [PubMed] [Google Scholar]
  4. Chang E. H., Gonda M. A., Ellis R. W., Scolnick E. M., Lowy D. R. Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4848–4852. doi: 10.1073/pnas.79.16.4848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Côté G. B. Reproductive drive and genetic counselling. Clin Genet. 1983 May;23(5):359–362. doi: 10.1111/j.1399-0004.1983.tb00446.x. [DOI] [PubMed] [Google Scholar]
  6. Dalla-Favera R., Bregni M., Erikson J., Patterson D., Gallo R. C., Croce C. M. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7824–7827. doi: 10.1073/pnas.79.24.7824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hall A., Marshall C. J., Spurr N. K., Weiss R. A. Identification of transforming gene in two human sarcoma cell lines as a new member of the ras gene family located on chromosome 1. Nature. 1983 Jun 2;303(5916):396–400. doi: 10.1038/303396a0. [DOI] [PubMed] [Google Scholar]
  8. Jeffreys A. J., Craig I. W., Francke U. Localisation of the G gamma-, A gamma-, delta- and beta-globin genes on the short arm of human chromosome 11. Nature. 1979 Oct 18;281(5732):606–608. doi: 10.1038/281606a0. [DOI] [PubMed] [Google Scholar]
  9. Kirsch I. R., Morton C. C., Nakahara K., Leder P. Human immunoglobulin heavy chain genes map to a region of translocations in malignant B lymphocytes. Science. 1982 Apr 16;216(4543):301–303. doi: 10.1126/science.6801764. [DOI] [PubMed] [Google Scholar]
  10. Malcolm S., Barton P., Murphy C., Ferguson-Smith M. A., Bentley D. L., Rabbitts T. H. Localization of human immunoglobulin kappa light chain variable region genes to the short arm of chromosome 2 by in situ hybridization. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4957–4961. doi: 10.1073/pnas.79.16.4957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Malcolm S., Barton P., Murphy C., Ferguson-Smith M. A. Chromosomal localization of a single copy gene by in situ hybridization--human beta globin genes on the short arm of chromosome 11. Ann Hum Genet. 1981 May;45(Pt 2):135–141. doi: 10.1111/j.1469-1809.1981.tb00315.x. [DOI] [PubMed] [Google Scholar]
  12. Marshall C. J., Hall A., Weiss R. A. A transforming gene present in human sarcoma cell lines. Nature. 1982 Sep 9;299(5879):171–173. doi: 10.1038/299171a0. [DOI] [PubMed] [Google Scholar]
  13. Marshall C. J., Kitchin R. M., Sager R. Genetic analysis of tumorigenesis: XII. Genetic control of the anchorage requirement in CHEF cells. Somatic Cell Genet. 1982 Nov;8(6):709–721. doi: 10.1007/BF01543013. [DOI] [PubMed] [Google Scholar]
  14. McBride O. W., Swan D. C., Santos E., Barbacid M., Tronick S. R., Aaronson S. A. Localization of the normal allele of T24 human bladder carcinoma oncogene to chromosome 11. Nature. 1982 Dec 23;300(5894):773–774. doi: 10.1038/300773a0. [DOI] [PubMed] [Google Scholar]
  15. Murray M. J., Cunningham J. M., Parada L. F., Dautry F., Lebowitz P., Weinberg R. A. The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell. 1983 Jul;33(3):749–757. doi: 10.1016/0092-8674(83)90017-x. [DOI] [PubMed] [Google Scholar]
  16. Neel B. G., Jhanwar S. C., Chaganti R. S., Hayward W. S. Two human c-onc genes are located on the long arm of chromosome 8. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7842–7846. doi: 10.1073/pnas.79.24.7842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. O'Brien S. J., Nash W. G., Goodwin J. L., Lowy D. R., Chang E. H. Dispersion of the ras family of transforming genes to four different chromosomes in man. Nature. 1983 Apr 28;302(5911):839–842. doi: 10.1038/302839a0. [DOI] [PubMed] [Google Scholar]
  18. Owerbach D., Bell G. I., Rutter W. J., Brown J. A., Shows T. B. The insulin gene is located on the short arm of chromosome 11 in humans. Diabetes. 1981 Mar;30(3):267–270. doi: 10.2337/diab.30.3.267. [DOI] [PubMed] [Google Scholar]
  19. Reddy E. P., Reynolds R. K., Santos E., Barbacid M. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene. Nature. 1982 Nov 11;300(5888):149–152. doi: 10.1038/300149a0. [DOI] [PubMed] [Google Scholar]
  20. Rowley J. D. Human oncogene locations and chromosome aberrations. Nature. 1983 Jan 27;301(5898):290–291. doi: 10.1038/301290a0. [DOI] [PubMed] [Google Scholar]
  21. Rowley J. D. Mapping of human chromosomal regions related to neoplasia: evidence from chromosomes 1 and 17. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5729–5733. doi: 10.1073/pnas.74.12.5729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sakaguchi A. Y., Naylor S. L., Shows T. B., Toole J. J., McCoy M., Weinberg R. A. Human c-Ki-ras2 proto-oncogene on chromosome 12. Science. 1983 Mar 4;219(4588):1081–1083. doi: 10.1126/science.6823569. [DOI] [PubMed] [Google Scholar]
  23. Shimizu K., Goldfarb M., Perucho M., Wigler M. Isolation and preliminary characterization of the transforming gene of a human neuroblastoma cell line. Proc Natl Acad Sci U S A. 1983 Jan;80(2):383–387. doi: 10.1073/pnas.80.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shimizu K., Goldfarb M., Suard Y., Perucho M., Li Y., Kamata T., Feramisco J., Stavnezer E., Fogh J., Wigler M. H. Three human transforming genes are related to the viral ras oncogenes. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2112–2116. doi: 10.1073/pnas.80.8.2112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Spira J., Wiener F., Babonits M., Gamble J., Miller J., Klein G. The role of chromosome 15 in murine leukemogenesis. I. Contrasting behavior of the tumor vs. normal parent-derived chromosomes No. 15 in somatic hybrids of varying tumorigenicity. Int J Cancer. 1981 Dec;28(6):785–798. doi: 10.1002/ijc.2910280618. [DOI] [PubMed] [Google Scholar]
  26. Stanbridge E. J., Der C. J., Doersen C. J., Nishimi R. Y., Peehl D. M., Weissman B. E., Wilkinson J. E. Human cell hybrids: analysis of transformation and tumorigenicity. Science. 1982 Jan 15;215(4530):252–259. doi: 10.1126/science.7053574. [DOI] [PubMed] [Google Scholar]
  27. Taparowsky E., Suard Y., Fasano O., Shimizu K., Goldfarb M., Wigler M. Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change. Nature. 1982 Dec 23;300(5894):762–765. doi: 10.1038/300762a0. [DOI] [PubMed] [Google Scholar]
  28. Taub R., Kirsch I., Morton C., Lenoir G., Swan D., Tronick S., Aaronson S., Leder P. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7837–7841. doi: 10.1073/pnas.79.24.7837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yuasa Y., Srivastava S. K., Dunn C. Y., Rhim J. S., Reddy E. P., Aaronson S. A. Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene. Nature. 1983 Jun 30;303(5920):775–779. doi: 10.1038/303775a0. [DOI] [PubMed] [Google Scholar]
  30. de Klein A., van Kessel A. G., Grosveld G., Bartram C. R., Hagemeijer A., Bootsma D., Spurr N. K., Heisterkamp N., Groffen J., Stephenson J. R. A cellular oncogene is translocated to the Philadelphia chromosome in chronic myelocytic leukaemia. Nature. 1982 Dec 23;300(5894):765–767. doi: 10.1038/300765a0. [DOI] [PubMed] [Google Scholar]
  31. de Martinville B., Giacalone J., Shih C., Weinberg R. A., Francke U. Oncogene from human EJ bladder carcinoma is located on the short arm of chromosome 11. Science. 1983 Feb 4;219(4584):498–501. doi: 10.1126/science.6297001. [DOI] [PubMed] [Google Scholar]

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