Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1983;2(12):2313–2318. doi: 10.1002/j.1460-2075.1983.tb01739.x

Regulation of the uvrC gene of Escherichia coli K12: localization and characterization of a damage-inducible promoter.

C A van Sluis, G F Moolenaar, C Backendorf
PMCID: PMC555450  PMID: 6321159

Abstract

Operon fusion and S1 nuclease mapping have been employed to locate a putative uvrC promoter, which is situated approximately 200 bp ahead of the uvrC structural gene. The promoter sustains transcription towards the uvrC coding sequence and is inducible by DNA damaging agents. The inducibility is dependent on the Escherichia coli LexA and RecA functions. Examination of the DNA sequence in the promoter region reveals the presence of a sequence similar to the consensus of a SOS box. In contrast to the related uvrA and uvrB genes, uvrC gene expression is characterized by a delayed onset of induction after DNA damaging treatment. Furthermore, no induction is observed with nalidixic acid.

Full text

PDF
2313

Images in this article

2315Fig. 5.
on p.2315

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Backendorf C., Brandsma J. A., Kartasova T., van de Putte P. In vivo regulation of the uvrA gene: role of the "-10" and "-35" promoter regions. Nucleic Acids Res. 1983 Sep 10;11(17):5795–5810. doi: 10.1093/nar/11.17.5795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bagg A., Kenyon C. J., Walker G. C. Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5749–5753. doi: 10.1073/pnas.78.9.5749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  4. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cole S. T. Characterisation of the promoter for the LexA regulated sulA gene of Escherichia coli. Mol Gen Genet. 1983;189(3):400–404. doi: 10.1007/BF00325901. [DOI] [PubMed] [Google Scholar]
  6. Fogliano M., Schendel P. F. Evidence for the inducibility of the uvrB operon. Nature. 1981 Jan 15;289(5794):196–198. doi: 10.1038/289196a0. [DOI] [PubMed] [Google Scholar]
  7. HOWARD-FLANDERS P., THERIOT L. A method for selecting radiation-sensitive mutants of Escherichia coli. Genetics. 1962 Sep;47:1219–1224. doi: 10.1093/genetics/47.9.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hanawalt P. C., Cooper P. K., Ganesan A. K., Smith C. A. DNA repair in bacteria and mammalian cells. Annu Rev Biochem. 1979;48:783–836. doi: 10.1146/annurev.bi.48.070179.004031. [DOI] [PubMed] [Google Scholar]
  9. Hentschel C., Irminger J. C., Bucher P., Birnstiel M. L. Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences. Nature. 1980 May 15;285(5761):147–151. doi: 10.1038/285147a0. [DOI] [PubMed] [Google Scholar]
  10. Kenyon C. J., Walker G. C. DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli. Proc Natl Acad Sci U S A. 1980 May;77(5):2819–2823. doi: 10.1073/pnas.77.5.2819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kenyon C. J., Walker G. C. Expression of the E. coli uvrA gene is inducible. Nature. 1981 Feb 26;289(5800):808–810. doi: 10.1038/289808a0. [DOI] [PubMed] [Google Scholar]
  12. Little J. W., Mount D. W. The SOS regulatory system of Escherichia coli. Cell. 1982 May;29(1):11–22. doi: 10.1016/0092-8674(82)90085-x. [DOI] [PubMed] [Google Scholar]
  13. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  14. McPartland A., Green L., Echols H. Control of recA gene RNA in E. coli: regulatory and signal genes. Cell. 1980 Jul;20(3):731–737. doi: 10.1016/0092-8674(80)90319-0. [DOI] [PubMed] [Google Scholar]
  15. Miki T., Kumahara H., Nakazawa A. Constriction of a fused operon consisting of the recA and kan (kanamycin resistance) genes and regulation of its expression by the lexA gene. Mol Gen Genet. 1981;183(1):25–31. doi: 10.1007/BF00270133. [DOI] [PubMed] [Google Scholar]
  16. Mount D. W. A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways. Proc Natl Acad Sci U S A. 1977 Jan;74(1):300–304. doi: 10.1073/pnas.74.1.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pannekoek H., Noordermeer I. A., van Sluis C. A., van de Putte P. Expression of the uvrB gene of Escherichia coli: in vitro construction of a pMB9 uvrB plasmid. J Bacteriol. 1978 Feb;133(2):884–896. doi: 10.1128/jb.133.2.884-896.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  19. Salles B., Paoletti C. Control of UV induction of recA protein. Proc Natl Acad Sci U S A. 1983 Jan;80(1):65–69. doi: 10.1073/pnas.80.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sancar A., Kacinski B. M., Mott D. L., Rupp W. D. Identification of the uvrC gene product. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5450–5454. doi: 10.1073/pnas.78.9.5450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sancar A., Rupp W. D. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region. Cell. 1983 May;33(1):249–260. doi: 10.1016/0092-8674(83)90354-9. [DOI] [PubMed] [Google Scholar]
  22. Sancar A., Sancar G. B., Rupp W. D., Little J. W., Mount D. W. LexA protein inhibits transcription of the E. coli uvrA gene in vitro. Nature. 1982 Jul 1;298(5869):96–98. doi: 10.1038/298096a0. [DOI] [PubMed] [Google Scholar]
  23. Sancar G. B., Sancar A., Little J. W., Rupp W. D. The uvrB gene of Escherichia coli has both lexA-repressed and lexA-independent promoters. Cell. 1982 Mar;28(3):523–530. doi: 10.1016/0092-8674(82)90207-0. [DOI] [PubMed] [Google Scholar]
  24. Schendel P. F., Fogliano M., Strausbaugh L. D. Regulation of the Escherichia coli K-12 uvrB operon. J Bacteriol. 1982 May;150(2):676–685. doi: 10.1128/jb.150.2.676-685.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Seeberg E. Multiprotein interactions in strand cleavage of DNA damaged by UV and chemicals. Prog Nucleic Acid Res Mol Biol. 1981;26:217–226. doi: 10.1016/s0079-6603(08)60406-7. [DOI] [PubMed] [Google Scholar]
  26. Sharma S., Dowhan W., Moses R. E. Molecular structure of uvrC gene of Escherichia coli: identification of DNA sequences required for transcription of the uvrC gene. Nucleic Acids Res. 1982 Sep 11;10(17):5209–5221. doi: 10.1093/nar/10.17.5209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sharma S., Moses R. E. uvrC gene function in excision repair in toluene-treated Escherichia coli. J Bacteriol. 1979 Jan;137(1):397–408. doi: 10.1128/jb.137.1.397-408.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sharma S., Ohta A., Dowhan W., Moses R. E. Cloning of the uvrC gene of Escherichia coli: expression of a DNA repair gene. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6033–6037. doi: 10.1073/pnas.78.10.6033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Smith C. L. recF-dependent induction of recA synthesis by coumermycin, a specific inhibitor of the B subunit of DNA gyrase. Proc Natl Acad Sci U S A. 1983 May;80(9):2510–2513. doi: 10.1073/pnas.80.9.2510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Smith G. R. DNA supercoiling: another level for regulating gene expression. Cell. 1981 Jun;24(3):599–600. doi: 10.1016/0092-8674(81)90085-4. [DOI] [PubMed] [Google Scholar]
  31. Strike P., Roberts R. J. Resident enhanced repair: novel repair process action on plasmid DNA transformed into Escherichia coli K-12. J Bacteriol. 1982 Apr;150(1):385–388. doi: 10.1128/jb.150.1.385-388.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Sugino A., Peebles C. L., Kreuzer K. N., Cozzarelli N. R. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767–4771. doi: 10.1073/pnas.74.11.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  34. Tang M., Lieberman M. W., King C. M. uvr Genes function differently in repair of acetylaminofluorene and aminofluorene DNA adducts. Nature. 1982 Oct 14;299(5884):646–648. doi: 10.1038/299646a0. [DOI] [PubMed] [Google Scholar]
  35. Yoakum G. H., Grossman L. Identification of E. coli uvrC protein. Nature. 1981 Jul 9;292(5819):171–173. doi: 10.1038/292171a0. [DOI] [PubMed] [Google Scholar]
  36. van den Berg E., Zwetsloot J., Noordermeer I., Pannekoek H., Dekker B., Dijkema R., van Ormondt H. The structure and function of the regulatory elements of the Escherichia coli uvrB gene. Nucleic Acids Res. 1981 Nov 11;9(21):5623–5643. doi: 10.1093/nar/9.21.5623. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES